山东医药2017，Vol.57Issue(25)：8-11,4.DOI:10.3969/j.issn.1002-266X.2017.25.003
甲基阿魏酸对CCl4诱导肝纤维化大鼠肝脏氧化应激损伤和细胞外基质积聚的影响
Effects of methyl ferulic acid on liver oxidative stress injury and extracellular matrix accumulation in rats with hepatic fibrosis induced by CCl4
摘要
Abstract
Objective To study the effects of methyl ferulic acid on liver oxidative stress injury and extracellular ma-trix accumulation in rats with liver fibrosis induced by carbon tetrachloride (CCl 4 ). Methods Sixty male SD rats were randomly divided into the normal group,model group,high-dose methyl ferulic acid group (20 mg/ kg),medium-dose methyl ferulic acid group (10 mg/ kg),and low-dose methyl ferulic acid group (5 mg/ kg). CCl 4 was used to replicate the liver fibrosis model,Methyl ferulic acid high-,medium-,and low-dose group were given the appropriate dose of methyl fer-ulic acid by gavage. Hepatic tissues were stained with HE and the pathological changes were observed. Biochemical analy-sis was used to detect the levels of alanine aminotransferase (ALT),aspartate aminotransferase (AST),catalase (CAT), glutathione peroxidase (GSH-Px),superoxide dismutase (SOD),and propane aldehyde (MDA). The content of serum precollagen Ⅲ (PCⅢ),type Ⅳ collagen (Ⅳ-C),hyaluronic acid (HA),and laminin (LN)was measured by enzyme-linked immunosorbent assay (ELISA). The expression of α-SMA,PCI mRNA and protein in liver tissues was detected by reverse transcription-polymerase chain reaction (RT-PCR)and Western blotting. Results Compared with the normal group,the levels of ALT,AST,MDA,PCⅢ,Ⅳ-C,HA,and LN of the model group increased significantly (all P <0. 05);the levels of CAT,GSH-Px,and SOD decreased significantly (P < 0. 05);liver and spleen index significantly in-creased (P < 0. 05);significant characteristics of liver fibrosis were found;the mRNA and protein expression of α-SMA and PCI in the liver tissues were significantly enhanced (both P < 0. 01). The levels of ALT,AST,MDA,PCⅢ,Ⅳ-C, HA,and LN decreased (all P < 0. 01),and the activities of CAT,GSH-Px,and SOD increased (all P < 0. 05),and the liver and spleen index significantly decreased (P < 0. 05),and the mRNA and protein expression of α-SMA and PCI in the liver tissues decreased in the high-,medium-,and low-dose groups as compared with those of the model group (all P <0. 01). Conclusions Methyl ferulic acid can effectively improve the liver oxidative stress injury,reduce the accumulation of extracellular matrix,and alleviate the degree of hepatic fibrosis of rats with hepatic fibrosis induced by CCl关键词
肝纤维化/甲基阿魏酸/四氯化碳/大鼠Key words
liver fibrosis/methyl ferulic acid/carbon tetrachloride/rats分类
医药卫生引用本文复制引用
李辰,黎莹,李丽,杨成芳,钟毓娟,吴丹,石琳,陈莉,李勇文..甲基阿魏酸对CCl4诱导肝纤维化大鼠肝脏氧化应激损伤和细胞外基质积聚的影响[J].山东医药,2017,57(25):8-11,4.基金项目
国家自然科学基金资助项目(81360497) （81360497）
广西高等学校优秀中青年骨干教师培养工程项目. （）
