山东医药2017，Vol.57Issue(26)：5-8,4.DOI:10.3969/j.issn.1002-266X.2017.26.002
成纤维细胞生长因子21对胰岛素抵抗肝细胞葡萄糖摄取的影响及机制
Effect of FGF-21 on glucose uptake of insulin-resistant liver cells
摘要
Abstract
Objective To observe the effect of fibroblast growth factors-21 (FGF21) on glucose uptake of insulin-resistant (IR) liver cells.Methods The fluorescently labeled FGF21 mRNA with certain stability was synthesized in vitro.The liver cells were cultured for 72 h in RPMI-1640 medium supplemented with 34.4 μmol/L recombinant insulin and 1 μmol/L dexamethasone to establish IR cell models, and then IR cell models were divided into the model control group, insulin group, and FGF21 group.We did not add any drugs into the model control group.The insulin group was treated with 1 μmol/L recombinant insulin and FGF21 group was transfected with FGF21 mRNA.The cell glucose uptake in the three groups was measured by glucose oxidase (GOD-POD) assay.The mRNA and protein expression of glucose transporter 1 (GLUT1) was detected by real-time fluorescent quantitative PCR and Western blotting.Results Compared with the model control group, the glucose uptake of the FGF21 group increased and the expression of GLUT1 mRNA and protein increased (all P<0.05).Compared with the model control group, the glucose uptake of the FGF21 group did not change significantly in the insulin group, and no significant difference was found in the GLUT1 mRNA and protein expression as compared with that of the model control group (all P>0.05).Conclusion FGF21 mRNA synthesized in vitro can improve the glucose uptake of IR liver cells, and its mechanism is related to the increase of GLUT1 expression.关键词
成纤维细胞生长因子21/葡萄糖转运蛋白1/体外转录mRNA/葡萄糖摄取/胰岛素抵抗/肝细胞Key words
fibroblast growth factors-21/glucose transporter 1/in vitro transcription of RNA/glucose uptake/insulin resistance/hepatic cells分类
生物科学引用本文复制引用
白洁,王金鑫,宋紫暄,吴志敏,高雁,朱泽,李光明..成纤维细胞生长因子21对胰岛素抵抗肝细胞葡萄糖摄取的影响及机制[J].山东医药,2017,57(26):5-8,4.基金项目
天津市科技计划项目(14ZCZDCX00054). （14ZCZDCX00054）
