山东医药2017，Vol.57Issue(26)：25-28,4.DOI:10.3969/j.issn.1002-266X.2017.26.007
miR-145靶向抑制MMP-9表达对大鼠脑动脉血管平滑肌细胞增殖和迁移的影响
Effects of microRNA-145 on proliferation and migration of rat arterial vascular smooth muscle cells through targeting MMP-9
摘要
Abstract
Objective To observe the effects of microRNA-145 on proliferation and migration of rat arterial vascular smooth muscle cells (VSMC) and its mechanism.Methods The rat brain basilar arterial VSMC was isolated by tissue patch assay.Then the cells were divided into three groups: the control group (transfection with empty carrier), miR-145 group (transfection with miR-145 mimics), and miR-145+MMP-9 group (transfection with miR-145 mimics and MMP-9).The mRNA and protein expression of MMP-9 was detected by qPCR and Western blotting, respectively.We added 50 ng/mL TNF-α to stimulate the cell proliferation, which was then examined by CCK8 assay.Transwell chamber was used to detect the cell migration ability.TargetScan and miRNAda identified 3′-UTR of MMP-9 mRNA had the target sequence of miR-145 in VSMCs.Luciferase reporter gene was used to validate the correlation between miR-145 and MMP-9.Results The MMP-9 mRNA and protein expression of the miR-145 group was significantly lower than that of the control group (both P<0.05), while the MMP-9 mRNA and protein expression in miR-145+MMP-9 group was higher than that of the miR-145 group (both P<0.05).After TNF-α treatment, OD value of the miR-145 group was lower than that of the control group, and OD value in miR-145+MMP-9 group was higher than that of miR-145 group (both P<0.05).Meanwhile, the miR-145 group had a significantly smaller number of migration cells than the control group, while the migration cells in miR-145+MMP-9 group were more than those of the miR-145 group (both P<0.01).Luciferase reporter revealed that miR-145 inhibited the relative luciferase activity of wild type MMP-9, but did not inhibit the mutant MMP-9.Conclusion MiR-145 inhibits the proliferation and migration of rat basilar arterial VSMC through down-regulating MMP-9.关键词
脑梗死/血管平滑肌细胞/微小RNA/miRNA-145/基质金属蛋白酶9/细胞增殖/细胞迁移Key words
cerebral infarction/vascular smooth muscle cell/microRNA/miR-145/matrix metalloproteinase 9/cell proliferation/cell migration分类
医药卫生引用本文复制引用
李文婷,邹伟,曹琳,张平..miR-145靶向抑制MMP-9表达对大鼠脑动脉血管平滑肌细胞增殖和迁移的影响[J].山东医药,2017,57(26):25-28,4.基金项目
湖南省医药卫生科研计划项目(C2016087). （C2016087）
