山东医药2017，Vol.57Issue(27)：23-26,4.DOI:10.3969/j.issn.1002-266X.2017.27.006
宫颈癌组织中maspin、AKT、p-AKT表达变化及其与肿瘤微淋巴管密度的相关性
Expression of maspin, AKT, and p-AKT in cervical carcinoma and its correlation with tumor lymphatic microvessel density
摘要
Abstract
Objective To observe the expression changes of maspin,protein kinase B (AKT),and phosphorylated protein kinase B (p-AKT) in the cervical cancer tissues and to analyze its correlation with tumor lymphatic microvessel density (LMVD).Methods Twenty-seven cases of cervical paraffin tissues were collected,including 20 cases of chronic cervicitis (inflammation group),20 cases of cervical intraepithelial neoplasia (CIN) Ⅰ (CIN1 group),20 cases of CIN Ⅱ (CIN2 group),20 cases of CIN Ⅲ (CIN3 group),and 47 cases of cervical cancer tissues (cervical cancer group,including 32 cases without lymph node metastasis of cervical cancer group 1 and 15 cases with lymph node metastasis of cervical cancer group 2).The levels of maspin,AKT,and P-AKT were measured by immunohistochemical SP method,and the LMVD was measured.Results The positive rate of maspin protein in the cervical cancer group 1 and cervical cancer group 2 was lower than that in the CIN3 group (all P<0.05).The positive expression rates of AKT and p-AKT in the cervical cancer group 1 and cervical cancer group 2 were higher than those in the other three groups (all P<0.05).The positive expression rate of p-AKT in the cervical cancer group 1 was higher than that in the cervical cancer group 2 (P<0.05).There was a negative correlation between the expression of maspin and AKT in the cervical cancer tissues (rs=-0.472,P=0.001).There was also a negative correlation between maspin and p-AKT expression (rs=-0.598,P=0.000).LMVD in the cervical cancer groups 1 and 2 were (19.44±2.95) and (23.24±2.46) vessels/mm2,respectively.There was a positive correlation between lymph node metastasis and LMVD (rs=0.44,P=0.000).There was a negative correlation between LMVD and the expression of maspin in the cervical cancer tissues (rs=-0.554,P=0.000).There was a positive correlation between LMVD and p-AKT expression (rs=0.39,P=0.007).Conclusions The expression of AKP and p-AKT is up-regulated in the cervical cancer tissues,and the expression of maspin is down-regulated,which is negatively correlated with both AKT and p-AKT expression.In the cervical cancer tissues,LMVD is negatively correlated with maspin expression,but is positively correlated with AKT expression.The down-regulation of maspin expression may be involved in the development of cervical cancer,and the mechanism may be related to the abnormal activation of AKT pathway and the promotion of tumor micro-lymphangion genesis.关键词
宫颈癌/maspin基因/蛋白激酶B/微淋巴管密度Key words
cervical cancer/maspin gene/protein kinase B/lymphatic microvessel density分类
医药卫生引用本文复制引用
许振,刘志强,孟玮,郭庆枝,孙莉..宫颈癌组织中maspin、AKT、p-AKT表达变化及其与肿瘤微淋巴管密度的相关性[J].山东医药,2017,57(27):23-26,4.基金项目
山东省自然科学基金资助项目(ZR2012HL01). （ZR2012HL01）
