山东医药2017，Vol.57Issue(27)：27-30,4.DOI:10.3969/j.issn.1002-266X.2017.27.007
40种中药提取物对6种临床常见感染菌株的体外抑菌活性观察
In vitro antimicrobial activity of 40 kinds of Chinese herbal extracts on six common clinical infectious strains
摘要
Abstract
Objective To observe the in vitro antibacterial activity of 40 kinds of common traditional Chinese medicine,such as Sumu (Sapanwood),Diyu(Sanguisorba officinalis),Yinyanghuo (Herba Epimedii),Huanglian (Rhizoma Coptidis),and Baishao (Radix Paeoniae Alba),etc.on the six common infectious strains.Methods Forty kinds of Chinese herbal medicine were selected to prepare the water extracts and ethanol extracts.Using the agar diffusion method (K-B method) to calculate the inhibitory zone diameter of 40 kinds of Chinese herb extracts on 6 standard strains (Staphylococcus aureus,Pseudomonas aeruginosa,Escherichia coli,Acinetobacter baumannii,Enterococcus faecalis,and Streptococcus pneumoniae) the antibacterial circle diameter.Screening out the Chinese herb extracts having antibacterial effect on standard strain (inhibition zone diameter range 10-21mm) of Chinese herbal extracts.Using broth microdilution method to test the minimum inhibitory concentration (MIC) of herbal extracts on six kinds of standard strains and clinical isolates.Results K-B showed that 15 kinds of Chinese herb extracts had inhibitory effects on at least one kind of standard strain,the diameter of inhibition zone was 10-21 mm.The MIC of 15 kinds of Chinese traditional medicine was 15.63-1 000 μg/mL.The MIC of Huanglian (Coptis chinensis) on streptococcus pneumoniae and Sumu (Sapanwood) on staphylococcus aureus was both 15.63 mg/mL.The MIC of Yinyanghuo (Epimedium) on streptococcus pneumoniae,Diyu (Sanguisorba) on streptococcus pneumoniae was 31.5 mg/mL.We selected 5 kinds of Chinese herbal medicine with strong antibacterial activity Sumu (Sapanwood),Diyu (Sanguisorba officinalis),Yinyanghuo (Herba Epimedii),Huanglian (Rhizoma Coptidis),Baishao (Radix Paeoniae Alba),which had different antimicrobial effects on 6 kinds of clinical isolates.The MIC50 of Sumu (Sapanwood) on methicillin-resistant staphylococcus aureus,and Huanglian (Rhizoma Coptidis) on ESBLs streptococcus pneumoniaeboth was both 15.63 mg/mL.Conclusions In 40 kinds of common traditional Chinese medicine,Sumu (Sapanwood),Diyu (Sanguisorba officinalis),Yinyanghuo (Herba Epimedii),Huanglian (Rhizoma Coptidis),and Baishao (Radix Paeoniae Alba) have the best in vitro antibacterial activity on the six common infectious strains.The inhibitory effect of Huanglian (Rhizoma Coptidis) on streptococcus pneumoniae and Sumu (Sapanwood) on staphylococcus aureus are the best.Sumu (Sapanwood) has a certain bacteriostatic effect on pseudomonas aeruginosa as well as Baishao (Radix Paeoniae Alba) and Diyu (Sanguisorba officinalis) on acinetobacter baumannii.关键词
中药/最小抑菌浓度/体外抑菌活性/中药提取物/药敏试验Key words
Chinese herbal medicine/minimal inhibitory concentration/antimicrobial activity in vitro/Chinese herb extracts/drug sensitive test分类
医药卫生引用本文复制引用
彭丹,马琼丽,陈泽慧,周小仙,陈安林,董泽令,杨欢,陈先恋..40种中药提取物对6种临床常见感染菌株的体外抑菌活性观察[J].山东医药,2017,57(27):27-30,4.基金项目
贵州省科技厅资助项目(黔科合-LH-2015-7480). （黔科合-LH-2015-7480）
