陕西中医2017，Vol.38Issue(6)：812-814,3.DOI:10.3969/j.issn.1000-7369.2017.06.059
不同白毛夏枯草提取物抗肝癌机制分析
Analysis of mechanism of anti-hepatoma different ajugadecumbens extractive
吴航 1令小峰2
作者信息
- 1. 青海省中医院,西宁810000
- 2. 西宁市食品药品监督管理局,西宁810008
- 折叠
摘要
Abstract
Objective:To mechanism of anti-hepatoma ajugadecumbens water extractive and ethyl acetate extract as an example to explore the different extracts.Methods:The water extract of Herba Ajugae (STW),ethyl acetate extract (ZTW) respectively in HepG2 and SMMC-7721 in hepatocellular carcinoma cell lines.Using four methyl thiazolyl tetrazolium (MTT) method was used to detect the inhibitory effect of different concentration of STW,ZTW and SMMC-7721 on HepG2 cell proliferation,apoptosis and apoptosis of hepatoma SMMC-7721 cells induced by HepG2 under different concentrations of STW and ZTW were detected by flow cytometry,using one-step fluorescent RT-PCR (Real-time PCR) expression technique for the detection of the different concentrations of STW,ZTW and HepG2 after treatment of SMMC-7721 cells in Bax mRNA.Results:STW and ZTW could significantly inhibit the proliferation and induce apoptosis of HepG2 and SMMC-7721 cells,and inhibit the proliferation of hepatoma cells.The inhibitory mechanism of STW and ZTW was concentration dependent,and the inhibitory effect of ZTW was the strongest.Conclusion:Herba Ajugae water extract and ethyl acetate extract by inhibiting proliferation of hepatoma cells,induce cell apoptosis,inhibit vascular proliferation,accelerate cell differentiation mechanisms against liver cancer.关键词
白毛夏枯草/提取物/抗肝癌机制Key words
Ajugadecumbens/Extractive/Mechanism of anti-hepatoma分类
医药卫生引用本文复制引用
吴航,令小峰..不同白毛夏枯草提取物抗肝癌机制分析[J].陕西中医,2017,38(6):812-814,3.
