同济大学学报（医学版）2017，Vol.38Issue(3)：14-18,24,6.DOI:10.16118/j.1008-0392.2017.03.003
CpG-ODN对肺癌A549细胞化疗的协同作用
CpG-ODN enhances sensitivity to chemotherapy in lung cancer cell line A549
摘要
Abstract
Objective To investigate the effect of TLR9 activator CpG-ODN on chemosensitivity of lung cancer A549 cells.Methods Cultured lung cancer A549 cells were divided into control group, DDP group, CpG-ODN group, DDP+CpG-ODN group, CpG-ODN+DDP group.Cell proliferation was analyzed with CCK-8 method;cell apoptosis was examined with flow cytometry;the mRNA expression of TLR9 was detected with RT-PCR;and the protein expression of MyD88 and AP-1 was detected with Western blotting.Results The cell proliferation was improved in CpG-ODN groups( P <0.05);there was no significant difference between CpG-ODN and CpG-ODN+DDP groups( P >0.05).The apoptosis rate of CpG-ODN+DDP was lower than that of DDP( P <0.05), and the apoptotic rate of DDP+CpG-ODN was significantly higher than that of DDP( P <0.05).The expression of TLR9 mRNA in DDP+CpG-ODN was higher than that in DDP group( P <0.05).The expression of AP-1 in DDP+CpG group was up-regulated( P <0.05).Conclusion The data indicate that the CpG-ODN can increase cell apoptosis and enhance the sensitivity of lung cancer cells to cisplatin chemotherapy.关键词
非小细胞肺癌/CpG寡聚脱氧核苷酸/化学疗法Key words
non-small cell lung cancer/CpG-ODN/chemotherapy分类
医药卫生引用本文复制引用
王凯玲,费广茹,任涛..CpG-ODN对肺癌A549细胞化疗的协同作用[J].同济大学学报（医学版）,2017,38(3):14-18,24,6.基金项目
国家自然科学基金(81372347) （81372347）
上海市浦东新区卫生系统重点学科建设项目(PWZx2014-10) （PWZx2014-10）
