同济大学学报（医学版）2017，Vol.38Issue(3)：19-24,6.DOI:10.16118/j.1008-0392.2017.03.004
β-catenin在小鼠下颌第一磨牙发育过程中的表达
Expression of β-catenin in mouse first mandibular molar tooth germs
摘要
Abstract
Objective To detect the expression pattern of β-catenin in the first mandibular molar tooth germs of mice and its relation to early tooth development.Methods Serial paraffin sections of the first mandibular molars at different development stages of mice(E11.5,E13.5,E14.5,E16.5,E18.5,PN0,PN3,PN7)were made.The expression of β-catenin was analyzed by immunohistochemistry.Results The expression patterns of β-catenin in the first lower molars varied among the mice with different development stages.It was positively expressed in the dental epithelium at tooth initiation stage;and it was detected in the central areas of the tooth bud during the bud stage, while the immunostaining was focused on the inner and outer enamel epithelium, especially on the enamel knot, during the cap stage.At the bell stage, the β-catenin was strongly expressed in the outer and inner enamel epithelium and the second enamel knot.During the early postnatal stage, the expression of β-catenin was detected in the areas where the ameloblasts or the odontoblasts were located.Conclusion The expression of β-catenin was in a temporal-spatial specific pattern;particularly, it is strongly expressed in the dental epithelial, enamel knot and odontoblasts with the development of tooth germ.This result indicates that-catenin may be involved in tooth morphogenesis of the mouse molars and the biological process of the odontoblast differentiation.关键词
β-catenin/Wnt信号通路/牙胚发育/磨牙/小鼠Key words
β-catenin/Wnt signaling pathway/tooth germ development/molar/mice分类
医药卫生引用本文复制引用
陈蔚婷,叶远舟,蔡昀,蒋备战..β-catenin在小鼠下颌第一磨牙发育过程中的表达[J].同济大学学报（医学版）,2017,38(3):19-24,6.基金项目
上海市科委医学引导项目(124119a7400、15411965800) （124119a7400、15411965800）
