同济大学学报（医学版）2017，Vol.38Issue(3)：40-45,6.DOI:10.16118/j.1008-0392.2017.03.008
内源性硫化氢对二乙基二硫代氨基甲酸盐诱导的大鼠慢性胰腺纤维化的影响
Effect of endogenous hydrogen sulfide on pancreatic fibrosis induced by diethyldithiocarbamate in rats
宋敏敏 1陈尼维 1伏桂香 1赵祥运 1范慧宁1
作者信息
- 1. 上海交通大学附属第六人民医院消化内科,上海 200233
- 折叠
摘要
Abstract
Objective To investigate the effect of sodium hydrogen sulfide (NaHS) on pancreatic fibrosis induced by diethyldithiocarbamate (DDC).Methods Forty-eight Wistar rats were randomized into four groups (12 per group): normal control group, model group, NaHS group and PAG group.Pancreatic fibrosis was induced by intraperitoneal injection with 10% DDC (700mg/kg);sodium hydrosulfide (56μmol/L) or DL-propargylglycine (45μmol/L) was intraperitoneally injected 1/d in rats of HaHS and PAG groups, respectively;an equal volume of saline was intraperitoneally injected in control and model groups.All animals were sacrificed on d30.The pancreas samples were harvested for the macroscopic and microscopic examinations.Collagen Ⅰ and TGF-β1 in pancreatic tissue were detected by ELISA method, Smad7 was determined by Western blotting, plasma H2S level was measured.Results Compared with the control group, the histological damage score, collagen area, the level of collagen Ⅰ and TGF-β1 were significant higher in model group, NaHS group and PAG group( P <0.05);those indicators in model group were significant higher than in NaHS group( P <0.05);those in PAG group were significant higher than in model group and NaHS groups( P <0.05).The expression of Smad7 was significant higher in control group than that in model group, NaHS group and PAG group( P <0.05);the expression of Smad7 in NaHS group was higher than that in model group and PAG group( P <0.05);the expression of Smad7 in model group was significant higher than that in PAG group( P <0.05).The level of serum H2S was significant higher in NaHS group than that in control group, model group and PAG group( P <0.05);the serum H2S level in control group was significant higher than that in model group( P <0.05);the serum H2S level in model group was significant higher than that in PAG group( P <0.05).Conclusion Hydrogen sulfide has a significant anti-fibrosis effect on pancreatic fibrosis induced by DDC in rats, which is possibly related to the up-regulation of the expression of Smad7 and down-regulation of the TGF-β1.关键词
胰腺纤维化/硫化氢/Ⅰ型胶原/转化生长因子-β1/Smad7蛋白/大鼠Key words
pancreatic fibrosis/hydrogen sulfide/collagen Ⅰ/transforming growth factor-β1/Smad7/rat分类
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宋敏敏,陈尼维,伏桂香,赵祥运,范慧宁..内源性硫化氢对二乙基二硫代氨基甲酸盐诱导的大鼠慢性胰腺纤维化的影响[J].同济大学学报（医学版）,2017,38(3):40-45,6.
