同济大学学报（医学版）2017，Vol.38Issue(3)：46-49,4.DOI:10.16118/j.1008-0392.2017.03.009
杜仲叶提取物对人精子膜功能损伤的保护作用
Eucommin protects human sperm membrane from oxidative injury
相俊 1卞崔冬 1万晓东 1吴登龙 1邵启蕙2
作者信息
- 1. 同济大学附属同济医院泌尿外科,上海 200065
- 2. 同济大学附属同济医院中医科,上海 200065
- 折叠
摘要
Abstract
Objective To investigate the protective effect of extraction of Eucommia ulmoides oliv (eucommin) on the function of human sperm membrane under oxidative injury.Methods The normal sperms were isolated with percoll gradient centrifugation, and the reactive oxygen species (ROS) were produced with hypoxanthine-xanzine xanzine (HX-XO) system.The human sperms suspension in ROS environment was treated with vitamin C (0.15mg/ml) or different concentrations of eucommin (150, 300 and 600mg/L).The malondialdehyde (MDA) content and superoxide dismutase (SOD) activity of sperm membranes were detected.The function of sperm membrane was analyzed by sperm hypotonic expansion test and the rate of intact acrosome was detected.Results Compared with oxidative model group, SOD activity of sperm suspension was improved,and the content of MDA was reduced in vitamin C group and eucommin groups ( P <0.01);the effect of high-dose eucommin group was more marked than that of vitamin C group ( P <0.01).There was significant difference in the rate of intact acrosome and sperm hypotonic expansion test between model group and eucommin groups ( P <0.01), and the high-dose eucommin group showed better result than other groups ( P <0.01).Conclusion Eucommin has a protective effect on human sperm membrane function from oxidative injury.关键词
精子/氧化损伤/杜仲/男性不育Key words
sperm/oxidative injury/Cortex Eucommiae/male infertility分类
医药卫生引用本文复制引用
相俊,卞崔冬,万晓东,吴登龙,邵启蕙..杜仲叶提取物对人精子膜功能损伤的保护作用[J].同济大学学报（医学版）,2017,38(3):46-49,4.
