生物加工过程2017，Vol.15Issue(4)：45-50,6.DOI:10.3969/j.issn.1672-3678.2017.04.008
"一锅双酶"法制备L-天冬氨酸的工艺条件优化
Optimizing of L-aspartic acid production by a "one-pot two-enzyme" process
摘要
Abstract
L-aspartic acid has been widely used in medicine,food and chemical industries.It is currently produced by a chemoenzymatic process at industrial scale.We have developed a "one-pot two-enzyme" process for the production of L-aspartic acid,which involves a maleate cis-trans isomerase and an l-aspartic acid amino lyase that are highly co-expressed in Escherichia coli.The fermentation conditions were optimized by orthogonal experimental design to improve the enzyme production.The optimum medium composition was as follows (g/L):tryptone 20,yeast extract 5,NaCl 5,fumaric acid 10,NH4NO3 8,lactose 6,and pH 7.5.The optimum conditions were as follow:rotational speed 150 r/min,ventilation 1.5 L/min,inoculum concentration 0.5%.The enzyme activity was two times higher than that before optimization,thus enabling the scale-up of the process to ensure the production of l-aspartic acid at industrial scale by this "one-pot two-enzyme" process.关键词
"一锅双酶"法/顺丁烯二酸/L-天冬氨酸/发酵工艺优化Key words
one-pot two-enzyme process/maleic acid/L-aspartic acid/fermentation optimization分类
化学化工引用本文复制引用
刘祥涛,张瑞,冯进辉,仪宏,吴洽庆,朱敦明,马延和.."一锅双酶"法制备L-天冬氨酸的工艺条件优化[J].生物加工过程,2017,15(4):45-50,6.基金项目
天津市科技计划(13ZCZDSY05200) （13ZCZDSY05200）
