生物技术通报2017，Vol.33Issue(8)：73-80,8.DOI:10.13560/j.cnki.biotech.bull.1985.2017-0395
山西大豆皂苷类型及其关键酶基因表达分析
Type Analysis of Saponin and Gene Expression of Key Enzyme in Shanxi Soybeans
摘要
Abstract
The contents of soysaponin Aa and Ab determine the taste of soybean products,this study aims to understand the composition and content of Aa and Ab soysaponin in soybean seeds,and then to study the mechanism of anabolism and synthesis. HPLC-ESI-MS/MS was employed to measure the contents of Aa and Ab saponins in soybean,and qRT-PCR technique to study the relative expression of GmSg-1 gene in grain accumulation. Results showed among 68 Shanxi soybean seeds,the range of Aa saponin variation were 0.41-33.79 mg/g and 0.21-8.39 mg/g in hypocotyls and cotyledons respectively for Aa type soybean materials ;and the range of Ab saponin variation were 0.85-44.96 mg/g and 0.26-11.09 mg/g for Ab type soybean materials. There were 19 SNP variants in GmSg-1 gene,and 10 of them resulting in amino acid substitutions were detected. The contents of Aa and Ab soysaponin increased at first and then decreased in the process of accumulation,and the contents of soysaponin were the highest after blossoming 40-50 d,while the relative expression of GmSg-1 gene was basically in the same trend as the content of soysaponin.关键词
大豆/皂苷/GmSg-1Key words
soybean/saponin/GmSg-1引用本文复制引用
赵巧玲,刁秀楠,郭春绒,赵晋忠,杜维俊,岳爱琴..山西大豆皂苷类型及其关键酶基因表达分析[J].生物技术通报,2017,33(8):73-80,8.基金项目
国家自然科学基金项目(31171580),山西省自然科学基金项目(201601D011076),山西省高等学校科技创新项目(2015148),山西农业大学中青年拔尖创新人才支持计划(201203),山西农业大学引进人才科研启动费(dwj) （31171580）
