生物技术通报2017，Vol.33Issue(8)：126-131,6.DOI:10.13560/j.cnki.biotech.bull.1985.2017-0236
Bt Cry8Ea蛋白对华北大黑鳃金龟幼虫(Holotrichia oblita)中肠微生物菌群的影响
Effects of Bt Cry8Ea Protein on the Midgut Microbial Flora of Holotrichia oblita Larvae
摘要
Abstract
Bt Cry8Ea protein presents insecticidal activity to Holotrichia oblita larvae. The interaction between Bt Cry8Ea protein and the midgut microorganism in H. oblita larvae was investigated by metagenome DNA analysis. The midguts in normal-fed or Cry8Ea protein-fed H. oblita(2nd-instar larva)were collected and then used to extract metagenome DNAs of midgut microorganism for constructing 16S rDNA gene libraries. Polymerase chain reaction restriction fragment polymorphism(PCR-RFLP)was employed to determine the polymorphisms of 16S rDNA enzyme-spliced fragments(Msp I,Rsa I,and Hae III restriction enzyme)from the midgut microorganism of two fed larvae. Different types of clones were sequenced and then aligned with NCBI gene database,and the phylogenetic tree was constructed to study the differences of microbial communities. The results showed that there were 30 bacteria in the normal group of H. oblita,and the predominant bacteria were Desulfosporosinus(29.63%),Clostridium(12.59%),and Bacillus(10.37%)respectively. However,there were only 4 bacteria in the Cry8Ea protein-fed H. oblita group,and the predominant bacteria were Enterococcus(50%)and Streptococcus(46.88%). These data revealed that the treatment with Cry8Ea protein changed the abundance and composition of midgut microbial flora.关键词
华北大黑鳃金龟/中肠微生物/BtCry8Ea蛋白/PCR-RFLPKey words
Holotrichia oblita/midgut microorganism/Bt Cry8Ea protein/PCR-RFLP引用本文复制引用
王伟,赵丹,郭巍,李新娜,张雅昆,闫晓平..Bt Cry8Ea蛋白对华北大黑鳃金龟幼虫(Holotrichia oblita)中肠微生物菌群的影响[J].生物技术通报,2017,33(8):126-131,6.基金项目
国家现代农业产业技术体系项目(CARS-14) （CARS-14）
