生物技术通报2017，Vol.33Issue(8)：132-138,7.DOI:10.13560/j.cnki.biotech.bull.1985.2017-0204
牦牛KLF3基因克隆及组织表达分析
Cloning and Tissue Expression Analysis of KLF3 Gene in Yak (Bos grunniens)
摘要
Abstract
The aims of this study are to clone yak KLF3 gene sequence,to analyze its biological characterization,and to clarify its expression patterns in different tissues. Six healthy,4-6 years old male Maiwa yaks were selected as experiment animals. After fasting slaughter,the tissue samples of spleen,lung,kidney,subcutaneous fat and longissimus dorsi muscle were collected for the total RNA extraction. The Reverse Transcription PCR(RT-PCR)was used to clone Maiwa yak KLF3 gene sequence,the quantitative real-time PCR(qPCR) was used to detect the expression levels of KLF3 gene in different tissues. The results showed that the sequence of yak KLF3 gene was 1137 bp (GenBank accession number :KX964630),containing 963 bp CDS,32 bp 5' UTR and 64 bp 3' UTR,encoding 346 amino acids. The amino acid sequence of yak KLF3 shared a similarity of 100% with that of cattle(XP_010820342.1). The mRNA expression level of KLF3 was high in lung and liver tissue,significantly higher than other tissues(P < 0.01).关键词
牦牛/KLF3基因/克隆/组织表达Key words
yak/KLF3 Gene/clone/tissue expression引用本文复制引用
林森,林亚秋,朱江江,柏雪,江明锋,王永..牦牛KLF3基因克隆及组织表达分析[J].生物技术通报,2017,33(8):132-138,7.基金项目
科技部科技支撑子项目(2014BAD13B03),中央高校基本科研业务费专项基金项目(2017NZYQN14) （2014BAD13B03）
