生物技术通报2017，Vol.33Issue(8)：152-158,7.DOI:10.13560/j.cnki.biotech.bull.1985.2017-0097
粟酒裂殖酵母中Mef2在线粒体中功能的研究
Function of Mef2 in Mitochondria of Schizosaccharomyces pombe
摘要
Abstract
Mitochondrial translation,essential for synthesis of the electron transport chain complexes in the mitochondria,is governed by nuclear encoded gene. This paper aims to reveal the main function of Mef2 protein in Schizosaccharomyces pombe. First,we constructed the strain of mef2 gene deletion by homologous recombination,and observed its growth phenotype in non-fermentative medium with glycerol as the sole carbon source. Second,the bioinformatics analysis demonstrated that the N-terminal of Mef2 contained a mitochondrial localization sequence(MTS)consisting of 31 amino acids. To further determine the localization of Mef2 protein,a GFP fluorescent label was added to the C-terminus of Mef2 to observe GFP green fluorescence. Third,Northern blotting was used to detect the effect of mef2 deletion on the level of mitochondrial genome encoding mRNAs. Finally,Western blotting was employed to detect the influence of the deletion of mef2 on the expression of mitochondrial genome-encoded protein. The results showed that Δmef2 strain caused growth defects on non-fermentation medium, which was one with mitochondrial respiratory defect. GFP green fluorescence localization experiments confirmed that Mef2 was localized in mitochondria. Northern blotting revealed that the deletion of mef2 did not affect the transcription of mRNAs encoded by the mitochondrial genome. Western blotting showed that the deletion of mef2 resulted in decrease in the expression of Cox1,Cox3,Atp6,and Cob1 proteins. In summary,Mef2 is a protein that is closely related to mitochondrial function and is involved in the translation of mitochondrial encoded proteins Cox1,Cox3,Atp6,and Cob1.关键词
线粒体/呼吸链/mef2/粟酒裂殖酵母Key words
mitochondrial/respiratory chain/mef2/Schizosaccharomyces pombe引用本文复制引用
张娟,陈美秀,商巾杰..粟酒裂殖酵母中Mef2在线粒体中功能的研究[J].生物技术通报,2017,33(8):152-158,7.基金项目
国家自然科学基金青年基金项目(31400032),江苏省高校自然科学研究项目(13KJB180010) （31400032）
