生物技术通报2017，Vol.33Issue(8)：174-179,6.DOI:10.13560/j.cnki.biotech.bull.1985.2017-0200
S-丙二酰转移酶基因失活对地中海拟无枝酸菌产利福霉素的影响
Effects of S-malonyltransferase Gene Inactivation on Rifamycin Production by Amycolatopsis mediterranei
何思雨 1成文玉 1金红星1
作者信息
- 1. 河北工业大学化工学院,天津 300130
- 折叠
摘要
Abstract
To increase rifamycin yield,the Amycolatopsis mediterranei mutant of the inactivated S-malonyltransferase gene(fabD) was constructed. S-malonyltransferase gene homologous recombinant vector was constructed using fusion PCR,and then transformed into A. mediterranei by electroporation,making the homologous recombination occurr. Then fabD-inactivated strain was screened by apramycin as a marker. Further,the rifamycin production between mutant strains was wmpared and the parental strain by fermentation. The results showed that the homologous recombinant vector of fabD was successfully constructed,and the A. mediterranei △ fabD was obtained ;the production of rifamycin SV in the mutant strain was 168.08 mg/L,with 9.94% increase in contrast to the parent strain. The inactivation of S-malonyltransferase gene weakened the fatty acid synthesis of mutant strain,subsequently strengthened the synthesis of rifamycin.关键词
地中海拟无枝酸菌/利福霉素/S-丙二酰转移酶/融合PCR/基因失活Key words
Amycolatopsis mediterranei/rifamycin/S-malonyltransferase/fusion PCR/gene inactivation引用本文复制引用
何思雨,成文玉,金红星..S-丙二酰转移酶基因失活对地中海拟无枝酸菌产利福霉素的影响[J].生物技术通报,2017,33(8):174-179,6.
