生物技术通报2017，Vol.33Issue(8)：199-205,7.DOI:10.13560/j.cnki.biotech.bull.1985.2017-0122
两种天然产物对B16F10细胞增殖及黑色素合成抑制机理研究
Study on Inhibition Mechanism of Melanogenesis and Melanin Synthesis of B16F10 Cell Induced by Two Natural Products
摘要
Abstract
This study aims to clarify the inhibition mechanism of proliferation and melanin synthesis of B16F10 cell induced by 10-hydroxycamptothecin(HCPT)and resveratrol(Res). MTT cytotoxicity assay,microscope observation-assay,L-Dopa oxidation-assay and NaOH cracking-assay were employed to investigate the proliferation,cellular morphology,tyrosinase(TYR)activity and melanin synthesis of B16F10 cells treated by HCPT and Res at different concentrations,respectively ;and semi-RT-PCR to analyze the relative mRNA expression of TYR and microphthalmia associated transcription factor(MITF)in B16F10 cells. It was found that HCPT(40,80,120,160, and 200 μmol/L)and Res(80,120,160,and 200 μmol/L)inhibited B16F10 cell proliferation through inducing apoptosis,and exhibited significantly dose-dependent inhibiting effects on tyrosinase activity and melanin synthesis on B16F10 cell(P < 0.05). In addition,relative mRNA expressions of the TYR and MITF genes in B16F10 cells were depressed remarkably by HCPT at different concentrations and Res at high concentrations(120 and 160 μmol/L). Conclusively,HCPT and Res may inhibit the B16F10 cell proliferation via inducing cell apoptosis, and depress the melanin synthesis in B16F10 cells from down-regulating the transcription of MITF gene and subsequently inhibiting the gene expression of TYR mRNA and activity of TYR.关键词
10-羟基喜树碱/白藜芦醇/酪氨酸酶/黑色素/小眼相关转录因子Key words
10-hydroxycamptothecin/resveratrol/tyrosinase/melanin/microphthalmia associated transcription factor引用本文复制引用
程杏安,张淑明,周晓武,吴波,林贤伟,秦湘静,黄素青,刘展眉,蒋旭红..两种天然产物对B16F10细胞增殖及黑色素合成抑制机理研究[J].生物技术通报,2017,33(8):199-205,7.基金项目
国家自然科学基金项目(21406274,21376281),广东省大学生创新训练项目(201411347034,201511347056) （21406274,21376281）
