山西农业大学学报（自然科学版）2017，Vol.37Issue(5)：326-329,365,5.
大麻THCA合成酶基因的克隆及生物信息学分析
Cloning and bioinformatics analysis of THCA synthase gene in hemp (Cannabis sativa L.)
摘要
Abstract
[Objective] The test was to obtain hemp strain which do not contain any THC.Cloning and bioinformatics analysis of THCA synthase gene could be helpful to facilitate the functions understanding of hemp THCA synthase.[Methods] The full-length THCA synthase cDNA sequence was cloned using RT-PCR from hemp variety "Huoma 1" and CsTHCA coding protein was analyzed by bioinformatics software,[Results] The open reading frame of CsTHCA was 1638bp in length and encoded 545 animo acids.The sequence homology analysis showed that CsTHCA protein had 74％ similarity with that of wild Arachis ipaensis and Arachis duranensis at the location of amino acids 309 bp to 760 bp.Physical and chemical properties analyzing revealed that this protein was hydrophobic protein with stable character.The analysis of protein structure and function domain indicated that this protein had transmembrane region,and most amino acids were hydrophobic.[Conclusion] The CsTHCA was cloned from hemp and was analyzed by bioinformatics.That could provide a theoretical basis for the study of the molecular mechanism.关键词
大麻/CsTHCA/基因克隆/生物信息学Key words
Cannabis sativa L/ CsTHCA/ Gene clone/ Bioinformatics analysis分类
农业科技引用本文复制引用
姜颖,潘冬梅,李秋芝,王晓楠,韩承伟,曹锟,韩喜财..大麻THCA合成酶基因的克隆及生物信息学分析[J].山西农业大学学报（自然科学版）,2017,37(5):326-329,365,5.基金项目
黑龙江省青年科学基金(QC2016037) （QC2016037）
