山西医科大学学报2017，Vol.48Issue(6)：632-635,4.DOI:10.13753/j.issn.1007-6611.2017.06.027
组织芯片技术在乳腺癌HER2 FISH检测中的可靠性分析
Analysis of the reliability of tissue microarrays in the research of HER2 FISH detection of breast cancer
陈玉娟 1张伟璇 1朱桂璐 1张伟1
作者信息
- 1. 皖南医学院弋矶山医院病理科,芜湖241001
- 折叠
摘要
Abstract
Objective To explore the reliability of tissue chip in the research of human breast cancer HER2 FISH detection.Methods Tissue microarray(TMA) with 0.9 mm in diameter tissue core was constructed from 192 cases of human breast cancer.HER2 was detected by FISH technique.At the same time the results of the tissue microarrays were compared with those of the routine paraffin section.Results The positive rates in HER2 FISH detection of breast cancer were 25.0％ and 26.0％ by TMA and the routine paraffin section,respectively,and there was no statistical difference (P ＞ 0.05).But the results of 4 cases were different between the two methods.Three cases of dot expansion using the routine paraffin section were negative by TMA,and 1 case of dot expansion using TMA showed no expansion by the routine paraffin section.Conclusion Tissue microarrays could be a reliable tool in the application of human breast cancer HER2 FISH detection,but it is better to use the routine paraffin section in the clinical.关键词
组织芯片/乳腺癌/HER2/FISHKey words
tissue microarrays/breast cancer/HER2/FISH分类
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陈玉娟,张伟璇,朱桂璐,张伟..组织芯片技术在乳腺癌HER2 FISH检测中的可靠性分析[J].山西医科大学学报,2017,48(6):632-635,4.
