现代检验医学杂志2017，Vol.32Issue(3)：37-39,3.DOI:10.3969/j.issn.1671-7414.2017.03.010
Gas6在免疫性血小板减少症患者中表达增高及临床意义
Increased Expression of Gas6 in Patients with Immune Thrombocytopenia and Its Clinical Significance
摘要
Abstract
Objective To investigate the expression level of Gas6 in patients with Immune Thrombocytopenia (ITP) and its clinical significance.Methods The experimental group was peripheral blood samples collected from 35 cases diagnosed with ITP in hematology department of Changhai Hospital in Shanghai from October 2013 to December 2015.Control group was peripheral blood from 35 healthy examined individuals at the same time.After separating plasm from the two group samples,the protein level of Gas6,IFN-α,IL-4,IFN-γ and IL-17 were measured by Enzyme-linked immunosorbent assay (ELISA),comparison of expressional level of the two groups was measured by t test.Pearson correlation analysis was used to decide the relation between Gas6 and cytokines such as IFN-α.Results The expression level of Gas6 in experimental and control groups was 27.28±7.56 ng/ml vs 20.51±5.39 ng/ml (t=4.314,P＜0.000 1);IFN-γ was 221.67±57.64 pg/ml vs 45.32 ±16.79 pg/ml (t=17.38,P＜0.000 1);IL-4 was 113.86±26.48 pg/ml vs 49.87±14.98 pg/ml (t=12.44,P＜0.000 1);IL-17 was 168.96±47.88 pg/ml vs 109.56±28.97 pg/ml (t=6.28,P＜0.000 1);IFN-α was 34.83±8.12 pg/ml vs 29.89 ± 5.76 pg/ml (t=2.936,P=0.004 5),all with statistical differences (P＜0.05).Pearson analysis showed that Gas6 was positively related with IL-17,IL-4,IFN γ (r=0.564,0.486,0.449,P＜0.05) and there was difference statistically,but Gas6 was not correlated with IFN-α.Conclusion Gas6 may participate in the disease formation of ITP through affection on cytokines secreted by Th cell subsets,and it was the potential therapeutic and predicted target for this disease clinically.关键词
免疫性血小板减少症/生长捕获特异性蛋白6/白细胞介素-4/干扰素-γ/白细胞介素-17Key words
Immune thrombocytopenia/Gas6/IL-4/IFN-γ/IL-17分类
医药卫生引用本文复制引用
李腾达,邓安梅,司宇,刘鹏,徐贵霞,谷明莉,叶辛,张薇薇,黄元兰,钱琤..Gas6在免疫性血小板减少症患者中表达增高及临床意义[J].现代检验医学杂志,2017,32(3):37-39,3.基金项目
973计划(2013CB531606),国家自然科学基金(81671556,81601406,81471605,81401358,81501397,31500721,81501398,81302579,81273282,81202353),上海申康基金(SHDC22014014),上海教育科学基金(D14017),军队科研基金(BWS14J023,15ZD009,15XD007),美捷登基金(MJR20150019). （2013CB531606）
