实用医学杂志2017，Vol.33Issue(11)：1757-1761,5.DOI:10.3969/j.issn.1006-5725.2017.11.010
IL-17、RORγt在百草枯中毒小鼠急性肺损伤中的表达
Roles of interleukin-17 and RORγt in a murine model with paraquat- induced acute lung injury
摘要
Abstract
Objective To explore the roles of Th17 cells in paraquat-induced acute lung injury(ALI)in a murine models through investigating the expression of Th17 type cytokine IL-17 and the specific transcription factor of Th17 cells-RORγt mRNA. Methods Male Kunming mice were randomly divided into the normal control group and the poisoned group. The ALI model induced by PQ poisoning was produced by intraperitoneal injection PQ solu-tion (20 mg/kg),and the normal control group was given normal saline solution with the same volume. After treatment for 48 hours,the animals were sacrificed. H&E staining and TUNEL staining were done to measure histo-logical changes and apoptosis. Serum level of Th17 related-cytokines was assayed by ELISA. The mRNA ex-pression of RORγt and the protein expression of IL-17 in the lung tissue were detected by real time-PCR assay and western blot,respectively. Results Compared with the normal control group,the lung inflammation and apopto-sis were significantly higher in the poisoned group. The serum level of IL-17A was significantly increased (P <0.05) and the lung parenchyma RORγt mRNA and IL-17 protein levels were significantly increased. Conclu-sion IL-17 plays an important role in the pathogenesis of ALI induced by paraquat poisoning.关键词
百草枯中毒/急性肺损伤/IL-17/炎症Key words
Paraquat poisoning/Acute lung injury/Interleukin-17/Inflammation引用本文复制引用
杨霞,张景鸿,陈威,李超乾..IL-17、RORγt在百草枯中毒小鼠急性肺损伤中的表达[J].实用医学杂志,2017,33(11):1757-1761,5.基金项目
广西急诊与医学救援人才小高地·广西高校急诊医学重点实验室课题(编号:GXJZ201503) （编号:GXJZ201503）
