实用医学杂志2017，Vol.33Issue(13)：2088-2092,5.DOI:10.3969/j.issn.1006-5725.2017.13.005
乙型肝炎病毒X蛋白对autotaxin表达的影响及其意义
Effect of hepatitis B virus X protein on autotaxin expression and its significance
摘要
Abstract
Objective To explore the effect of hepatitis B virus (HBV) X protein (HBx) on autotaxin (ATX) expression and its significance. Methods The recombinant eukaryotic expression vector of HBx ,pcD-NA3.1(+)-HBx,and the recombinant luciferase reporter gene vector of ATX promoter,pGL3-ATX,were con-structed and used to co-transfect HepG2 cells to examine the effect of HBx on the activity of ATX promoter. The sta-ble cell expressing HBx,HepG2.HBx,was constructed,and Western blot(WB)was used to detect the effect of HBx on ATX expression. Results The luciferase activity of pcDNA3.1(+)-HBx and pGL3-ATX group was 1.47 times as that of the empty vector cDNA3.1(+)and pGL3-ATX group(P<0.000). WB detection showed that the expression of ATX protein was increased in HepG2.HBx cells,and 1.75 times as that of HepG2 cells(P<0.05). Conclusion HBx can activate ATX promoter and up-regulate ATX expression ,thus suggests that HBV infection might enhance ATX/LPA signaling.关键词
乙型肝炎病毒/X蛋白/Autotaxin/基因表达/Autotaxin/溶血磷脂酸信号转导Key words
Hepatitis B virus/X protein/autotaxin/gene expression/autotaxin/lysophosphatidic acid(LPA)signaling引用本文复制引用
肖雅伦,苏何玲,谭燕莲,梁斌,谷云艳,莫之婧,黄红丽,刘永明..乙型肝炎病毒X蛋白对autotaxin表达的影响及其意义[J].实用医学杂志,2017,33(13):2088-2092,5.基金项目
国家自然科学基金项目(编号:81560341, 81460320) （编号:81560341, 81460320）
广西研究生教育创新计划项目(编号:JGY2015129) （编号:JGY2015129）
