实用医学杂志2017，Vol.33Issue(14)：2271-2274,4.DOI:10.3969/j.issn.1006-5725.2017.14.006
TGF-βRⅡsiRNA对AngⅡ诱导GMC增殖的影响
Effect of TGF-βRⅡ siRNA on AngⅡ induced proliferation of glomerular mesangial cell
摘要
Abstract
Objective To investigate the effect of transforming growth factor β receptor-Ⅱ(TGF-βRⅡ) on AngⅡ inducing glomerular mesangial cell(GMC) proliferation and the expressions of TGF-β1 and psmad3 through transfecting TGF-βRⅡsmall interfering RNA(siRNA)into GMC. Methods Through transfecting fluo-rescence control siRNA into GMC ,we observed the transfection efficiency under fluorescence microscope after 6 hours. Transfecting TGF-βRⅡsiRNA and negative control siRNA into GMC respectively ,we detected the expres-sion of TGF-βRⅡ by western blot after 24 hours. The cells were divided into four groups:control group,AngⅡgroup,negative siRNA control group and TGF-βRⅡ siRNA group. Each group was stimulated by AngⅡ except the control group. After 24 hours,we detected the TGF-β1 and psmad3 protein levels by western blot and detected GMC proliferation by CCK8 kit. Results (1) Comparing with the scrambled control group,the expression of TGFβRⅡin the TGF-βRⅡsiRNA group was significantly decreased(P<0.05).(2)AngⅡcould accelerate the expression of TGF-β1. Transfecting TGF-βRⅡsiRNA into GMC decreased the expression of TGF-β1 protein(P<0.05).(3)AngⅡ could stimulate the phosphorylation of smad3. Transfecting TGF-βRⅡ siRNA into GMC de-creased the expression of psmad3 protein(P < 0.05).(4)Transfecting TGF-βRⅡ siRNA into GMC relieved the GMC proliferation AngⅡ-promoted(P < 0.05). Conclusions The AngⅡ stimulates the GMC proliferation,de-pending on the expression of TGF-βRⅡ. It is related to the expressions of TGF-β1 and psmad3.关键词
TGF-βRⅡ/TGF-β1/血管紧张素Ⅱ/肾小球系膜细胞Key words
TGF-βRⅡ/TGF-β1/angiotensinⅡ/GMC引用本文复制引用
谢燕媚,任静,黄启丽,纪泽泉..TGF-βRⅡsiRNA对AngⅡ诱导GMC增殖的影响[J].实用医学杂志,2017,33(14):2271-2274,4.基金项目
广东省教育厅特色创新项目(编号:2014KTSCX 100) （编号:2014KTSCX 100）
广州市科技计划项目(编号:201510010200) （编号:201510010200）
