天津中医药2017，Vol.34Issue(7)：491-494,4.DOI:10.11656/j.issn.1672-1519.2017.07.17
松脂醇二葡萄糖苷大鼠胆汁内排泄研究
Study on bile excretion of pinoresinol diglucoside of rats
摘要
Abstract
[Objective] To establish a method for simultaneous determination of pinoresinol diglucoside and its metabolites pinoresinol singleglucoside in rat bile by liquid chromatography-mass spectrometry (LC-MS/MS) and to study the excretion of two compound from rat bile. [Methods] Bile samples were collected according to the designed time periods after intragastric administration. The content of pinoresinol diglucoside, with the warfarin prime as internal standard, and it's metabolites in rat bile were then analyzed using the established LC-MS/MS method. [Results] Calibration curves were generated over a concentration range of 8~5000 ng/mL for pinoresinol diglucoside and pinoresinol singleglucoside, respectively. Interday , intra-day and other methodology study RSDs were in accordance with the requirements of the biological samples quantitatively. Biliary accumulated excretion after administration were growing continually, to maintain a steady state after 6 hours;discharge rate in 4 hours to reach the top, afterdischarge rate falling rapidly, after 6 hours in a low condition,rats excretion rate has a rasing trend in the late experiment. [Conclusion] This method meets the requirements for the quantitative determination of biological samples and can be used forquantitative analysis of pinoresinol diglucoside and it's metabolites pinoresinol singleglucosidein rat bile.关键词
松脂醇二葡萄糖苷/液质联用/胆汁排泄/代谢产物Key words
pinoresinol diglucoside/LC-MS/MS/biliary excretion/metabolite分类
医药卫生引用本文复制引用
韩亚亚,王磊,张文杰,郝佳,刘二伟..松脂醇二葡萄糖苷大鼠胆汁内排泄研究[J].天津中医药,2017,34(7):491-494,4.基金项目
高等学校博士学科点专项科研基金项目(20131210120010) （20131210120010）
国家科技重大专项(2013ZX09401004) （2013ZX09401004）
天津市科技创新体系及条件平台建设计划项目(14TXZYJC00440). （14TXZYJC00440）
