天然产物研究与开发2017，Vol.29Issue(6)：924-928,982,6.DOI:10.16333/j.1001-6880.2017.6.004
白杨素与咖啡酸苯乙酯抗肿瘤活性比较
Comparison of Anti-tumor Activity of Chrysin and Caffeic Acid Phenethyl Ester
摘要
Abstract
To study the anti-tumor activities of chrysin and caffeic acid phenethyl ester (CAPE).The normal cultured human breast cancer (MCF-7,MDA-MB-231),human cerrical cancer cells (HeLa),human lung carcinoma (A549) and human umbilical vein endothelial cells (HUVECs) were treated with different concentrations of chrysin and caffeic acid phenethyl ester (20,40,80,160 μM) for 24 and 48 h,respectively.The cell viability was measured by SRB method.Hoechst 33258 staining and acridine orange staining were used to observe apoptotic morphology and nuclear fragmentation,respectively.The cell migration was detected by scratch method.Intracellular reactive oxygen species (ROS) level and mitochondria memberane potential were determined by utilizing a fluorescent probe,2',7'-dichlorodihydrofluorescin (DCHF) and JC-1,and the level of NF-κB p65 was tested by immunofluorescence.The results showed that chrysin and CAPE inhibited different tumor cells proliferation and migration,and upregulated ROS level and downregulated mitochondrial membrane potential and NF-κB p65 levels.Chrysin and caffeic acid phenethyl ester were potential anti-tumor agents.关键词
白杨素/咖啡酸苯乙酯/抗肿瘤Key words
chrysin/caffeic acid phenethyl ester/anti-tumor分类
医药卫生引用本文复制引用
王月华,常化松,尹旭升,刘新迎,玄红专..白杨素与咖啡酸苯乙酯抗肿瘤活性比较[J].天然产物研究与开发,2017,29(6):924-928,982,6.基金项目
国家自然科学基金(31672499) （31672499）
山东省高等学校科技计划(J16LE21) （J16LE21）
山东省农业创新体系(SDAIT-24-05) （SDAIT-24-05）
