天然产物研究与开发2017，Vol.29Issue(6)：934-940,7.DOI:10.16333/j.1001-6880.2017.6.006
地榆总皂苷对60Co-γ射线所致骨髓抑制小鼠外周血象的保护作用及其机制
Protective Effects of Peripheral Blood of Total Saponins of Sanguisorba officinalis L.on Myelosuppressive Mice induced by 60Co-γ ray and Its Associated Mechanism
摘要
Abstract
Protective effects and mechanism of total saponins of Sanguisorba officinalisL.(TSSO) on myelosuppres-sivemiceinduced by 60Co-γ ray was investigated.The myelosuppressivemice modelwas established by irradiating the whole body one time with 650 cGy60Co-γ ray.Then the myelosuppressive mice were treated with TSSO(1,0.5 and 0.1 mg/kg,i.g.) once a day,for total 10 days.The levels of WBC,RBC,HCT,PLT and PCT were measured at the 4th,7th and 10th day.The next day,the liver,spleen and thymus was collected for counting organ index.The numbers of bone marrow cells were also counted and the mRNA expressions of GM-CSF,IL-3,SCF and c-Kit were measured by qPCR analysis.The results showed that TSSO can improve the levels of WBC,RBC,HCT,PLT,PCT,BMC,and the mRNA expression of GM-CSF,IL-3,SCF,and c-Kit in TSSO groups significantly.Thus,TSSO can alleviate myelosuppression induced by 60Co-γ ray.The improvement of the mRNA expressions of GM-CSF,IL-3,SCF and c-Kit may be the mechanisms.关键词
地榆总皂苷/60Co-γ射线/骨髓抑制/IL-3/GM-CSF/SCF/c-KitKey words
total saponins of Sanguisorba officinalisL./6oCo-γ ray/myelosuppression/IL-3/GM-CSF/SCF/c-Kit分类
医药卫生引用本文复制引用
朱林杰,陈琳,黄飞鸿,代燕平,邹文俊,吴建明..地榆总皂苷对60Co-γ射线所致骨髓抑制小鼠外周血象的保护作用及其机制[J].天然产物研究与开发,2017,29(6):934-940,7.基金项目
四川省科技厅项目(14JC0798) （14JC0798）
四川省教育厅重点项目(15ZA0155) （15ZA0155）
泸州市科技计划(2015-S-43) （2015-S-43）
西南医科大学附属医院项目(15114) （15114）
