天然产物研究与开发2017，Vol.29Issue(6)：941-946,6.DOI:10.16333/j.1001-6880.2017.6.007
哈蟆油通过激活PI3K/Akt/NF-κB途径保护氧化应激损伤的大鼠卵巢颗粒细胞
Protective Effect of Oviductus Ranae on Oxidative Stress-induced Injury in Rat Ovarian Granulosa Cells by Activation of PI3K/Akt/NF-κB Signal Transduction Pathway
摘要
Abstract
The aim of this study was to investigate the function and mechanism of Oviductus Ranae (OR) on protecting rat ovarian granulosa cells from H2O2-induced oxidative damage.Granulosa cells were divided into six groups:control,model,positive control,as well as low,middle and high doses of OR-containing serum group.The granulosa cells were exposed to H2O2 for 2 h,and then treated with the OR-containing serum for 48 h.After that,the viability of granulosa cells was detected by CCK-8 assay.mRNA expressions of PI3K and AKT3 were assayed by real-time qPCR.The related proteins,such as NF-κB (p65),IκBα,IKKβ,and p-Akt (Ser473),were detected by Western blotting.The results showed that the viability of granulosa cells was significantly decreased after stimulating H2O2.OR-containing serum can significantly increase the viability of granulosa cells.Furthermore,OR-containing serum can rescue the descending expression of PI3K and AKT3 mRNA induced by H2O2,and up-regulate the protein levels of p-Akt (Ser473),IKKβ and NF-κB (p65),and down-regulate the expression of IκBα.These results indicated that OR protected granulosa cells from oxidative stress-induced injury by regulating the PI3K/Akt/NF-κB signal pathway,which may be one of the antioxidant mechanism of OR.关键词
哈蟆油/大鼠卵巢颗粒细胞/氧化应激/H2O2Key words
Oviductus Ranae/ovarian granulosa cells/oxidative stress/H2O2分类
医药卫生引用本文复制引用
零小妹,张绪慧,杨晶晶,吴新荣,梁磊..哈蟆油通过激活PI3K/Akt/NF-κB途径保护氧化应激损伤的大鼠卵巢颗粒细胞[J].天然产物研究与开发,2017,29(6):941-946,6.基金项目
广州市珠江科技新星项目(2013J2200030) （2013J2200030）
广州市科技计划(201509010012) （201509010012）
