天然产物研究与开发2017，Vol.29Issue(6)：1000-1005,1019,7.DOI:10.16333/j.1001-6880.2017.6.017
冬凌草提取物对金黄色葡萄球菌生物膜形成的抑制作用
Inhibition Activity of Rabdosia rubescens Extract on Biofilm Formation of Staphylococcus aureus
摘要
Abstract
The effect of Rabdosia rubescens extract on biofilm formation of Staphylococcus aureus was tested.Inhibitory activity of R.rubescens extract with different concentrations and different addition times on the biofilm formation of S.aureus were analyzed by crystal violet staining assay and sliver staining methods.The results showed that the MIC and MBC of R.rubescens crude extract against S.aureus were 125 μg/mL and 250 μg/mL,respectively.Under sub-MIC concentrations,the intervention effect of R.rubescens extract on biofilm formation of S.aureus was not obvious,but when the concentration of R.rubescens crude extracts was more than double MIC,it can significantly inhibit the biofilm formation of S.aureus and the best inhibition effect can be achieved after adding drugs at the initial time of biofilm formation.The inhibition rate was over 79％ when the concentrations of R.rubescens crude extracts were 2MIC.The inhibition rate was only 60％ at 24 h of biofilm formation after adding drugs.The eradication effect on biofilm and the inhibition of extracellular polysaccharide(EPS) synthesis were studied by using crystal violet staining assay and sliver staining methods.The results also showed that the eradication effect of R.rubescens crude extracts on biofilm and the inhibition of its on extracellular polysaccharide synthesis were weak.An obvious decrease of S.aureus biofilms and EPS were observed after adding drugs at tbe initial time of biofilm formation when the concentration of R.rubescens crude extracts was more than double MIC.This study provided a theoretical basis for the wide application of R.rubescens extract in food storage and preservation,as well as antibiotic-free feed.关键词
冬凌草/金黄色葡萄球菌/生物膜/抑菌活性Key words
Rabdosia rubescens/Staphylococcus aureus/biofilm/antibacterial activity分类
轻工纺织引用本文复制引用
汪伦记,许美秋,纠敏,原江锋,赵亚美..冬凌草提取物对金黄色葡萄球菌生物膜形成的抑制作用[J].天然产物研究与开发,2017,29(6):1000-1005,1019,7.基金项目
河南省教育厅重点项目(14B180019) （14B180019）
