生态环境学报2017，Vol.26Issue(5)：778-784,7.DOI:10.16258/j.cnki.1674-5906.2017.05.008
西瓜连作对热区土壤微生物多样性的影响
Response of Soil Microbial Diversities to Continuous Watermelon Cropping in Tropical Region in China
摘要
Abstract
In the modern agricultural production, continuous cropping obstacle is always one big problem to us. Many ways were attempted, but there were no effective methods to overcome continuous cropping obstacles. We have investigated the application of RAPD technology in tropical soil microbial diversity of watermelon continuous cropping. In order to provide scientific basis for solving the problem of watermelon continuous cropping obstacle. Screening and optimization of primers for RAPD, screened 18 RAPD random primers were used to amplify the watermelon root rhizosphere soil DNA. A total of 154 bands were amplified, of which 154 (100%) were polymorphic: the average was 8.56 per primer. RAPD technique was used to analyze the changes of soil microbial diversity and soil community in the rhizosphere and non rhizosphere of different cropping during the four growth stages. The changes of soil microbial communities in the same cropping during the four growth stages were also analyzed by RAPD. The results showed that the changes of microbial diversity were larger both in the rhizosphere and non rhizosphere soil during the four growth stages. The variation coefficients of richness index, Dsh and Jsh were more than 29%. Single factor analysis showed that the effects of continuous cropping on microbial diversity were significant in rhizosphere and non rhizosphere (P<0.05). Further analysis showed that the sampling temperature was positively correlated with the rhizosphere and non rhizosphere microbial diversity index, and the correlation coefficient was more than 0.82. Soil microbial diversity decreased significantly in the rhizosphere soil of continuous watermelon cropping. In different growth stages, the response of rhizosphere microbial diversity was different. The soil microbial diversity had the greatest influence on soil microbial diversity during the second - third period, and the variation coefficient was 22.43% and 15.53%. In early fruiting stage of the second cropping, the Response of microbial diversity was maximum. Early fruiting stage of the third cropping was second only to early fruiting stage of the second cropping. The coefficients of variation were 22.43% and 15.53%.关键词
连作/西瓜/土壤微生物多样性/RAPDKey words
continuous cropping/watermelon/soil microbial diversities/RAPD分类
农业科技引用本文复制引用
吴宇佳,谢良商,张冬明,吉清妹,郑道君..西瓜连作对热区土壤微生物多样性的影响[J].生态环境学报,2017,26(5):778-784,7.基金项目
海南省自然科学基金项目(312082) （312082）
