西安交通大学学报（医学版）2017，Vol.38Issue(3)：457-461,封3,6.DOI:10.7652/jdyxb201703025
龙葵素通过ROS/p38信号通路诱导人前列腺癌细胞凋亡
Solanine induces the apoptosis of human prostate cancer cells via ROS/p38 signaling pathway
摘要
Abstract
Objective To investigate the molecular mechanism of solanine-induced apoptosis of prostate cancer cells Du145 and LNCaP.Methods The effects of solanine on the viability of Du145 and LNCaP cells were evaluated by MTT assay.The generation of intracellular reactive oxygen species (ROS) and solanine-induced apoptosis were measured by flow cytometry.The protein levels of p38 and p-p38 expressions were examined by Western blot.Results Solanine significantly inhibited the viability of Du145 and LNCaP cells in a dose-dependent manner (P＜0.01).The inhibition of solanine on cell viability was suppressed by the ROS scavenger NAC.ROS generation,apoptosis and phosphorylation of p38 were induced by treatment with solanine at 40 μmol/L for 24 h.The expression of p38 and solanine-induced apoptosis were suppressed by NAC and SB203580.Conclusion Solanine induces the apoptosis of human prostate cancer cell via the RO.S-p38 signaling pathway.关键词
龙葵素/细胞凋亡/前列腺癌/p38Key words
solanine/apoptosis/prostate cancer/p38分类
医药卫生引用本文复制引用
钟伟枫,陈南辉,黄裕清,万沛,林毅锋,江惠明,钟凯华,潘斌,刘思平..龙葵素通过ROS/p38信号通路诱导人前列腺癌细胞凋亡[J].西安交通大学学报（医学版）,2017,38(3):457-461,封3,6.基金项目
广州市医药卫生科技项目(No.20141A010105) （No.20141A010105）
广东省自然科学基金-博士启动项目(No.2015A030310250) （No.2015A030310250）
中国博士后科学基金面上项目(No.2016M602595)Supported by Medical and Health Science and Technology Project of Guangzhou (No.20141A010105),the Natural Science Foundation of Guangdong Province-Doctoral Startup Project (No.2015A030310250),and China Postdoctoral Science Foundation Grant (No.2016M602595) （No.2016M602595）
