西北林学院学报2017，Vol.32Issue(4)：69-72,110,5.DOI:10.3969/j.issn.1001-7461.2017.04.12
巴山木竹蛋白质可见/近红外光谱定量分析模型研究
Quantitative Visible/Near-infrared Spectroscopy Analysis Models of the Protein in Bashania fargesii
摘要
Abstract
The feasibility to establish quantitative analysis models of the protein in Bashania fargesii by applying visible/near-infrared (Vis/NIR) spectroscopy was studied.The content of protein in each sample was analyzed by the Kjeldahl method and calibration models of PLS,PCR between protein content and spectral data were established by spectrum analysis software.The optimal calibration models were selected by the main performance indexes,different spectrum datum pretreatment methods were also studied and compared.Prediction abilities of optimal calibration models were tested by the samples in validation set.The correlation coefficients (Rc2) of the calibration models of the protein in B.fargesii leaves and culms were 0.935 and 0.862,respectively.The root-mean-square error of calibration (RMSEC) were 0.351 and 0.172,respectively.The correlation coefficients of prediction (R2p) were 0.916 and 0.874,RPD (SD/RMSECV) of validation set were 3.562 and 2.840,both were greater than 2.5.The results indicated that Vis/NIR spectroscopy could be used in the fast determination of the protein in B.fargesii.关键词
巴山木竹/蛋白质/可见/近红外光谱/定量分析Key words
Bashania fargesii/protein/visible/near-infrared spectroscopy/quantitative analysis分类
农业科技引用本文复制引用
王逸之,董文渊..巴山木竹蛋白质可见/近红外光谱定量分析模型研究[J].西北林学院学报,2017,32(4):69-72,110,5.基金项目
国家林业局公益性行业科研专项(201204103) （201204103）
西南林业大学科研启动基金项目(111440) （111440）
云南省科技惠民计划项目(2013CA003). （2013CA003）
