西北林学院学报2017，Vol.32Issue(4)：81-86,6.DOI:10.3969/j.issn.1001-7461.2017.04.14
基于转录组数据的月季SSR标记开发及花色遗传性分析
Development of SSR Markers Based on the Transcriptome Data of Rose and Analysis of Flower Color Inheritance
摘要
Abstract
On the basis of the transcriptomic data of rose,the SSR primers were developed with plant materials of R.laxa,R,rugosa'White',R.cv.'Pink Peace',R.multi flora Thunb.var.albo-plena and R.xanthina Lindl f.normalis,which had far relationship between each other.The genetic diversity of F1 group hybridized from R.cv.'Funkuhr'×R.cv.'Red France'was evaluated by using these developed primers.The results were as follows:1)80 pairs of SSR primers had designed,among which 44 were with target fragment,besides,28 were with polymorphism.2)The genetic distance of F1 group was between 0.52 and 0.94.3) Clustering analysis showed that F1 group at genetic distance 0.52 was divided into two categories,one was with their female parent'Funkuhr'(HT-13),in which the flower color was generally yellow,the other was with male parent'Red France'(HT-12),in which the color was generally between red and pink.The results suggested that the cause of the color separation was mainly associated with color inheritance,that the color red was the dominant character to yellow.The color separation might also associated with metabolism of flavonoids and anthocyanin.关键词
月季/SSR/引物开发/遗传多样性/花色分离Key words
rose/SSR/molecular marker development/genetic diversity/color separation分类
农业科技引用本文复制引用
张非亚,罗玉兰,有祥亮,贾桂霞,张冬梅..基于转录组数据的月季SSR标记开发及花色遗传性分析[J].西北林学院学报,2017,32(4):81-86,6.基金项目
上海市科委科技攻关项目“耐湿热月季育种与种质创制”(13391900902). （13391900902）
