西北林学院学报2017，Vol.32Issue(4)：147-152,6.DOI:10.3969/j.issn.1001-7461.2017.04.26
软枣猕猴桃EST-SSR引物开发及通用性研究
Development and Transferability of EST-SSR Primers in Actinidia arguta
摘要
Abstract
EST-SSR primers with the number of 196 pairs of Actinidia arguta were developed to screen the transferability of the primers to 25 Actinidia accessions,also cluster analysis of the 25 accessions was constructed.Thirty five pairs of EST-SSR primers were screened out.A total of 189 bands were amplified,with a mean of 5.40bands per primer.The products were 170-250 bp,in which the polymorphic bands were 178 (94％),indicating the genetic diversity of Actinidia was high,and the A.arguta EST-SSR primers was highly transferable too.Cluster analysis indicated that the genetic similarity coefficient (GS) of 25 Actinidia accessions ranged from 0.591 to 0.969.At the similarity coefficient of 0.70,A.chinensis and A.deliciosa were classified as one branch,showed the close relationship between the two species.关键词
软枣猕猴桃/EST-SSR/多态性/通用性/聚类分析Key words
Actinidia arguta/EST-SSR/polymorphism/transferability/cluster analysis分类
农业科技引用本文复制引用
王丹丹,杨东霞..软枣猕猴桃EST-SSR引物开发及通用性研究[J].西北林学院学报,2017,32(4):147-152,6.基金项目
国家自然科学基金(31370400) （31370400）
丹东市2016科技攻关项目(2016KJ001),辽东学院青年基金项目(2015QN006). （2016KJ001）
