陕西科技大学学报（自然科学版）2017，Vol.35Issue(4)：132-137,6.
B16细胞与HaCaT细胞体外共培养模型的建立
Construction of the co-culture model of B16cells and HaCaT cells in vitro
摘要
Abstract
The co-culture model of Murine Melanoma cells (B16 cells) and human keratinocytes (HaCaT cells) was established,and the effect of the model by arbutin and 8-MOP.B16 cells and HaCaT cells were cultured in 10% DMEM medium.Different inoculation sequences of cell and inoculation concentrations of cell were used to construct co-culture model.After arbutin and 8-MOP were added to the model,the cytotoxicity,melanin content and tyrosinase activity were measured to verify the reliability of the model.The results showed that the sequences of cell inoculation had no effect on the co-culture model;B16 cells was co-culture with HaCaT cells as 1∶4 ratio;the highest inhibitory effect of arbutin on the melanin content and tyrosinase activity at the concentration of 100 μg / ml;the 8-MOP had the lowest inhibitory effect on melanin content and tyrosinase activity at the concentration of 20 μg / ml,all of these like as the previous study of only B16 cells.The co-culture model of B16 cells and HaCaT cells was established effectively in this study and it can be used to screen the drugs of promoting or inhibiting melanin.关键词
B16细胞/HaCaT细胞/共培养模型/熊果苷/8-MOPKey words
B16 cells/HaCaT cells/co-culture/arbutin/8-MOP分类
生物科学引用本文复制引用
朱祯慧,朱丽清,陈金妹,林娇芬,潘裕添..B16细胞与HaCaT细胞体外共培养模型的建立[J].陕西科技大学学报（自然科学版）,2017,35(4):132-137,6.基金项目
福建省科技厅青年科技创新项目(2015J05071) （2015J05071）
