现代妇产科进展2017，Vol.26Issue(7)：485-488,4.DOI:10.13283/j.cnki.xdfckjz.2017.07.001
CTHRC1对卵巢癌细胞顺铂耐药的作用及机制研究
The role of CTHRC1 in cisplatin-resistance of ovarian cancer cells and related mechanisms
摘要
Abstract
Objective:To determine the effect of collagen triple helix repeat containing-1 (CTHRC1) on cisplatin-resistance of ovarian cancer cells and related mechanisms.Methods:The expression of CTHRC1 in A2780、A2780/DDP、SKOV3、SKOV3/DDP cells was detected by Western blot.SKOV3/DDP cells were transfected with lenti-shCTHRC1 to down-regulate CTHRC1.Then CCK-8 kit was used to investigate the capability of proliferation and the half-cell lethal dose (IC50) of cisplatin.The expression of Bcl-2,p-STAT3,p-Akt were assessed by Western blot.Result:The expression of CTHRC1 in A2780、SKOV3 cells was lower than that in A2780/DDP、SKOV3/DDP cells (P<0.05).Down-regulation of CTHRC1 had no influence on the proliferation of SKOV3/DDP cells,however,it cloud significantly decreased the IC50 of cisplatin in SKOV3/DDP cells (P<0.01).Meanwhile,the phosphorylation of Akt、STAT3 was inhibited and the expression of Bcl-2 was down-regulated in SKOV3/DDP-shCTHRC1 cells (P<0.05).Conclusions:CTHRC1 may regulate Akt、STAT3/BcL-2 signaling to enhance the cisplatin-resistance in ovarian cancer cells.关键词
CTHRC1/卵巢肿瘤/顺铂/耐药Key words
CTHRC1/Ovarian cancer/Cisplatin/Drug-resistance分类
医药卫生引用本文复制引用
郭碧莹,李露莹,张殊..CTHRC1对卵巢癌细胞顺铂耐药的作用及机制研究[J].现代妇产科进展,2017,26(7):485-488,4.基金项目
国家自然科学基金资助项目(No:81672564) （No:81672564）
