现代妇产科进展2017，Vol.26Issue(7)：489-492,4.DOI:10.13283/j.cnki.xdfckjz.2017.07.002
O-GLcNAc糖基化介导的β-catenin水平变化对卵巢癌细胞增殖的影响
β-catenin enhanced the ability of cell proliferation via its O-GLcNAcylation in ovarian cancer cell
马春 1张玮 2王建3
作者信息
- 1. 第四军医大学西京医院妇产科,西安 710032
- 2. 宁夏军区医院妇产科,银川 750004
- 3. 宁夏医科大学,银川 750004
- 折叠
摘要
Abstract
Objective:To investigate the influence of the change of β-catenin induced by O-GLcNAcylation in ovarian cancer cells.Methods:SKOV3 cell was transfected with lentivirus to bulid O-GLcNAc lower expression model.A2780 was treated with PUGNAc,a inhibitor of O-GLcNAc hydrolase (OGA),to bulid O-GLcNAc higher expression model.Fowcytometry technology was used to test cell cycle.MTT was used to test cell proliferation.Western blot was used to test β-catenin expression in different cell models.CO-IP was used to test the glycosylation level of β-catenin in different cell models.Results:Cell proliferation was influenced by the level of O-GLcNAcylation.The levels of O-GLcNAcylation mediated the expression of β-catenin in ovarian cancer cell.Conclusion:High levels of O-GLcNAcylation can enhance the expression of β-catenin,and thus influence cell proliferation in ovarian cancer cell.关键词
卵巢癌/O-GlcNAc/β-catenin/细胞增殖Key words
Ovarian cancer/O-GlcNAc/β-catenin/Cell proliferation分类
医药卫生引用本文复制引用
马春,张玮,王建..O-GLcNAc糖基化介导的β-catenin水平变化对卵巢癌细胞增殖的影响[J].现代妇产科进展,2017,26(7):489-492,4.
