现代妇产科进展2017，Vol.26Issue(7)：502-505,4.DOI:10.13283/j.cnki.xdfckjz.2017.07.005
辣椒碱对人宫颈癌细胞Hela和C-33A的抗肿瘤活性
Anti-tumor activity of capsaicin on human cervical cancer cell Hela and C-33A
徐妍 1刘建华2
作者信息
- 1. 上海交通大学医学院附属第九人民医院妇产科,上海 200011
- 2. 上海中医药大学附属第七人民医院妇产科,上海 200137
- 折叠
摘要
Abstract
Objective:To observe the capsaicin on Hela (HPV+) and C-33A cell growth inhibition and influence on cell cycle and cell apoptosis.To explore the effects of capsaicin on cervical cancer cell inhibitory effect and the related mechanism.Methods:Two kinds of cervical cancer cell lines,HPV positive Hela and HPV negative C-33A cells,were chosen.Cell activity were examined by CCK-8 method,the inhibition of cell growth,cell cycles and cell apoptosis after capsaicin treatment were examined by flow cytometry.Cell apoptosis related gene mRNA and protein expression were detected by qPCR and Western blot.Results:The inhibitions of growth in Hela and C-33A cells after capsaicin treatment was getting severer along with the capaicin concentration increase and time prolongation.The capsaicin could promote the apoptosis of both kinds of cancer cells,and even more apoptosis of Hela cells.The expressions of cytochrom C,Caspase3 and bax were significantly increased,and the expression of Bcl-2 was significantly decreased.Conclusions:Capsaicin could effectively inhibit the growth and promote the apoptosis of Hela and C-33A.The anti-tumor effects of capsaicin might be achieved by cytchrome C pathway.Specifically,capsaicin may induce ROS increase in Hela and C-33A cell,then cytchrome C relive from mitochondria and trigere Caspase 3 apoptosis pathway in cell.关键词
辣椒碱/宫颈癌细胞/抗肿瘤/凋亡Key words
Capsaicin/Cervical cell lines/Antitumor activity/Apoptosis分类
医药卫生引用本文复制引用
徐妍,刘建华..辣椒碱对人宫颈癌细胞Hela和C-33A的抗肿瘤活性[J].现代妇产科进展,2017,26(7):502-505,4.
