现代妇产科进展2017，Vol.26Issue(7)：521-524,4.DOI:10.13283/j.cnki.xdfckjz.2017.07.010
叉头框F2在宫腔粘连中的表达及意义
Expression and significance of forkhead box F2 in intrauterine adhesions
摘要
Abstract
Objective:To establish a stable cell model of intrauterine adhesions and to detect the expression of forkhead box F2(FoxF2).Methods:Human endometrial stromal cells(HESCs) cultured by primary method were identified by immunofluorescence staining.Applied 0,2.5,5,10ng/ml transforming growth factor β1(TGF-β1) to HESCs for 48 hours and then detected the mRNA and protein expressions of α-smooth muscle actin(α-SMA),collagen I(COLI) and FoxF2 by PCR and Western blot.Applied 10ng/ml TGF-β1 to HESCs for 12,24,48,72hours and then detected the mRNA and protein expressions of α-SMA,COLI and FoxF2 by same methods.Results:The mRNA and protein expressions of α-SMA,COLI and FoxF2 were increased gradually in the groups of 2.5,5ng/ml and 10ng/ml compared with the 0ng/ml group.As the effect time of 10ng/ml TGF-β1 was prolonged,the mRNA and protein expressions of α-SMA,COLI,and FoxF2 were also increased gradually in the groups of 24,48 and 72hours compared with 12 hours group.Conclusion:TGF-β1 can induce the fibrosis of HESCs,thus the cell model of intrauterine adhesions was established.The expressions of FoxF2 increased significantly in intrauterine adhesions.关键词
子宫内膜基质细胞/宫腔粘连/纤维化/转化生长因子β1/叉头框F2Key words
Endometrial stromal cells/Intrauterine adhesions/Fibrosis/Transforming growth factor β1/Forkhead box F2分类
医药卫生引用本文复制引用
陈思萍,何援利,刘丽敏,蔡慧华,孙冬华,张冬梅..叉头框F2在宫腔粘连中的表达及意义[J].现代妇产科进展,2017,26(7):521-524,4.基金项目
国家自然科学基金资助(No:81270658) （No:81270658）
