中国组织工程研究2017，Vol.21Issue(20)：3123-3128,6.DOI:10.3969/j.issn.2095-4344.2017.20.002
射频联合富血小板血浆修复半月板白区撕裂
Radiofrequency combined with platelet-rich plasma for repair of white meniscal tears
摘要
Abstract
BACKGROUND: Both biological and physical methods contribute to the repair of white meniscal tears, so the combined use will be better. OBJECTIVE: To study the influence of radiofrequency combined with platelet-rich plasma (PRP) on the repair of white meniscal tears. METHODS: Forty-eight adult New Zealand white rabbits were randomly divided into four groups, including simple suture, radiofrequency, PRP, and combination groups, and then the model of medial meniscus posterior root tears was established in all experimental animals. The simple suture group underwent interrupted mattress suture; the radiofrequency group was treated with radiofrequency (20 W, 45 ℃) after suture; the PRP group received the intra-articular injection of PRP after suture; the combination group was firstly treated with radiofrequency after suture, and then underwent the intra-articular injection of PRP. The gross and histological changes of the meniscus were observed, and the expression levels of transforming growth factor β1, vascular endothelial growth factor and platelet-derived growth factor were detected at 3 and 12 weeks postoperatively. RESULTS AND CONCLUSION: At 12 weeks after operation, the simple suture group showed no healing; the radiofrequency and PRP groups healed partially; and the combination group healed completely, and chondrocytes and collagen fibers arranged regularly. There was a significant difference in the healing rate between combination and simple suture groups (P=0.003). At 3 weeks postoperatively, the expression levels of vascular endothelial growth factor in the radiofrequency, PRP and combination groups were obviously higher than that in the simple suture group, which decreased markedly at the 12th week. At 3 weeks postoperatively, the expression level of platelet-derived growth factor was increased in all groups, especially in the PRP and combination groups. The PRP group showed the highest level of transforming growth factor β1 at 12 weeks postoperatively. These results manifest that the combination of radiofrequency and PRP promotes the repair of white meniscal tears by increasing the cell proliferation, cell mitosis and angiogenesis.关键词
组织构建/组织工程/射频/富血小板血浆/半月板/修复/撕裂/白区/细胞因子分类
医药卫生引用本文复制引用
周伟峰,朱琳,姜雪峰,王亚斌..射频联合富血小板血浆修复半月板白区撕裂[J].中国组织工程研究,2017,21(20):3123-3128,6.基金项目
江苏省卫生科研项目(Z201217) （Z201217）
江苏省科技计划项目(澄政科[2011]85 号社-22)the Project of Health Scientific Research of Jiangsu Province, No. Z201217 （澄政科[2011]85 号社-22）
the Science and Technology Project of Jiangsu Province, No. (2011)85-22 （2011）
