中国组织工程研究2017，Vol.21Issue(20)：3129-3134,6.DOI:10.3969/j.issn.2095-4344.2017.20.003
红车轴草提取物对破骨细胞骨吸收及分化的作用机制
Effect of Trifolium pratense L. extracts on the bone resorption and osteogenic differentiation of osteoclasts and the underlying mechanisms
李扬 1王德平 2艾东 3魏鑫 3关利新 3关悦2
作者信息
- 1. 牡丹江医学院附属红旗医院,骨科,黑龙江省牡丹江市 157011
- 2. 牡丹江医学院附属红旗医院,内分泌科,黑龙江省牡丹江市 157011
- 3. 牡丹江医学院,黑龙江省牡丹江市 157011
- 折叠
摘要
Abstract
BACKGROUND: Isoflavone isolated from Trifolium pratense L. has been found to be able to effectively inhibit bone resorption, reduce bone turnover rate, improve osteocyte activity and bone mineral density by enhancing the effect of estrogen, which is helpful for the prevention and treatment of osteoporosis. OBJECTIVE: To investigate the effect of Trifolium pratense L. extracts on the bone resorption and differentiation of osteoclasts.METHODS: Rat bone marrow cells were extracted, isolated by lymphocyte separation and cultured for 5 hours; then, the non-adherent cells were selected followed by induced by 30 μg/L macrophage colony stimulating factor and 75 μg/L RANKL (control groups), or different concentrations of Trifolium pratense L. extracts (0.3, 0.6 and 1.2 g/L) to observe their effect on the osteoclast differentiation and bone resorption. The levels of osteoclast differentiation-associated proteins c-fos and NFATcl were determined by western blot assay. RESULTS AND CONCLUSION: Compared with the control group, different concentrations of Trifolium pratense L. extracts could suppress osteoclast differentiation and bone resorption to different degrees. Tartrate-resistant acid phosphatase staining showed that Trifolium pratense L. extracts could significantly reduce the number of osteoclasts. Western blot assay results suggest that Trifolium pratense L. extracts significantly inhibited the expression levels of c-fos and NFATcl. These results reveal that Trifolium pratense L. extracts can inhibit osteoclast differentiation and bone resorption.关键词
组织构建/骨组织工程/骨质疏松/破骨细胞分化/骨吸收/抗酒石酸酸性磷酸酶/骨陷窝/NFATC1/c-fos/红车轴草分类
医药卫生引用本文复制引用
李扬,王德平,艾东,魏鑫,关利新,关悦..红车轴草提取物对破骨细胞骨吸收及分化的作用机制[J].中国组织工程研究,2017,21(20):3129-3134,6.
