中国组织工程研究2017，Vol.21Issue(20)：3135-3140,6.DOI:10.3969/j.issn.2095-4344.2017.20.004
H-型血管在骨关节炎软骨下骨中的表达及作用
Expression and role of H-type vessels in the subchondral bone in the progression of osteoarthritis
摘要
Abstract
BACKGROUND: H-type vessels are mainly distributed in the metaphysis, which can promote the proliferation of osteocytes, further accelerating osteogenesis.OBJECTIVE: To investigate the expression of H-type vessels in the subchondral bone during the occurrence and development of osteoarthritis.METHODS: 8-week-old C57 mice were randomly divided into experimental and sham groups, followed by the right medial menisectomy to establish the osteoarthritis models or only articular capsulotomy. The knee samples were removed at 4 weeks postoperatively, and were stained with safranin-O-fast green to evaluate the degree of injury. The expression levels of CD31, Emcn and matrix metalloproteinase 13 were detected by immunofluorescent staining. The changes of the subchondral bone were observed by hematoxylin-eosin staining and the changes of bone mass in the subchondral bone were analyzed by micro-CT.RESULTS AND CONCLUSION: Compared with the sham group, the Osteoarthritis Research Society International scores, expression levels of CD31, Emcn, H-type vessels and matrix metalloproteinase 13, as well as the bone mass in the subchondral bone were significantly increased in the experimental group (P < 0.05). To conclude, the increased H-type vessels in the subchondral bone promote the hyperplasia and remodeling of subchondral bone in the progression of osteoarthritis.关键词
组织构建/骨组织工程/骨关节炎/软骨下骨/血管/H-型血管/国家自然科学基金分类
医药卫生引用本文复制引用
卢键森,柳鑫,曾春,赵畅,方航,蔡道章..H-型血管在骨关节炎软骨下骨中的表达及作用[J].中国组织工程研究,2017,21(20):3135-3140,6.基金项目
国家自然科学基金(81371990) （81371990）
国家自然科学基金(81601945) （81601945）
高等学校博士学科点专项科研基金(20124433110021) （20124433110021）
广东省医学科研基金(A2015422)the National Natural Science Foundation of China, No. 81371990 and 81601945 （A2015422）
the Doctoral Special Fund of Universities, No. 20124433110021 （）
the Medical Research Foundation of Guangdong Province, No. A2015422 （）
