中国组织工程研究2017，Vol.21Issue(20)：3164-3169,6.DOI:10.3969/j.issn.2095-4344.2017.20.009
不同剂量维甲酸诱导骨质疏松大鼠模型的稳定性
Different dosages of retinoic acid to establish a rat model of osteoporosis: a stability evaluation
摘要
Abstract
BACKGROUND: Osteoporosis is a serious threat to the health and quality of life in the elderly. It is important to establish an ideal experimental animal model to study the etiology and treatment of osteoporosis.OBJECTIVE: To establish a rat model of osteoporosis induced by different dosages of retinoic acid, thus selecting the optimal dosage.METHODS: Eighty female Sprague-Dawley rats were randomly divided into control, low-, middle- and high-dosage groups based on body mass (n=20 per group), The rats in the latter three groups were induced with 80, 100, and 120 mg/(kg?d) retinoic acid via gastric lavage for 14 days.RESULTS AND CONCLUSION: Compared with the control group, the bone mineral density, number of osteoblasts and osteoclasts, and bone microarchitecture in the low-dosage group showed no significant changes, while there were significant decrease in the serum level of calcium and bone mineral density of femur, significant increase in the number of osteoclasts at the femur and significant changes in the femoral microarchitecture in the middle- and high-dosage groups, especially in the middle-dose group. To conclude, 120 mg/(kg?d) retinoic acid via gastric lavage for 14 days can induce a stable osteoporosis model in rats.关键词
组织构建/骨组织工程/骨软骨损伤与修复动物模型/骨质疏松/雌性/维甲酸/苏木精-伊红染色/骨组织形态计量学分类
医药卫生引用本文复制引用
孙世栋,梁其彬,范为之,曾展鹏,陈柏行..不同剂量维甲酸诱导骨质疏松大鼠模型的稳定性[J].中国组织工程研究,2017,21(20):3164-3169,6.基金项目
广东省科技计划项目(2014A020212593)the Science and Technology Program of Guangdong Province, No. 2014A020212593 （2014A020212593）
