中国组织工程研究2017，Vol.21Issue(20)：3183-3189,7.DOI:10.3969/j.issn.2095-4344.2017.20.012
腺病毒介导神经生长因子和髓磷脂相关糖蛋白双基因在受损坐骨神经中的表达
Expression of adenovirus-mediated nerve growth factor and myelin-associated glycoprotein double-gene in sciatic nerve injury
摘要
Abstract
BACKGROUND: Nerve growth factor (NGF) and myelin-associated glycoprotein (MAG) can promote the repair of peripheral nerve. However, increasing the expression of NGF and MAG in the injured peripheral nerve by transgene technology has not been reported.OBJECTIVE: To investigate the expression of NGF and MAG after adenovirus (Ad) mediated transfection in the injured sciatic nerve in rats.METHODS: Forty Sprague-Dawley rats were randomly divided into normal, model, Ad, NGF and NGF-MAG groups. Sciatic nerve injury models were established in the latter four groups by cutting and suturing the right sciatic nerve. Afterwards, the rats in each group were subject to the intramuscular injection of normal saline, normal saline, Ad (1×108 PFU), Ad-NGF (1×108 PFU), and Ad-NGF-MAG (1×108 PFU), respectively, once every 2 days, for consecutive three times.RESULTS AND CONCLUSION: The expression level of NGF in the rat sciatic nerve in the Ad and normal groups was significantly higher than that in the NGF and NGF-MAG groups (P < 0.05). Compared with the Ad, NGF and normal groups, the expression level of MAG in the NGF-MAG group was significantly increased (P < 0.05). To conclude, the Ad-mediated NGF and MAG after double gene transfection were expressed effectively in the injured sciatic nerve in rats.关键词
组织构建/组织工程/坐骨神经损伤/神经生长因子/髓磷脂相关糖蛋白/腺病毒/神经修复/周围神经/基因技术/国家自然科学基金分类
医药卫生引用本文复制引用
陈渝,邓忠良,陈诗谋,翁政,黄方..腺病毒介导神经生长因子和髓磷脂相关糖蛋白双基因在受损坐骨神经中的表达[J].中国组织工程研究,2017,21(20):3183-3189,7.基金项目
国家自然科学基金项目(81272005) （81272005）
重庆市北碚区科委重点课题(2008-10)the National Natural Science Foundation of China, No. 81272005 （2008-10）
the Key Project of Chongqing Beibei District Science Committee, No. 2008-10 （）
