中国组织工程研究2017，Vol.21Issue(21)：3281-3286,6.DOI:10.3969/j.issn.2095-4344.2017.21.001
硫化氢通过缺氧诱导因子1α促进张应力下骨髓间充质细胞的成骨
Hydrogen sulfide promotes the osteogenesis of bone marrow mesenchymal cells through hypoxia-inducible factor-1 alpha under tensile stress
摘要
Abstract
BACKGROUND: Hydrogen sulfide signaling has been proved to promote distraction osteogenesis; however,the underlying mechanism remains unclear.OBJECTIVE: To evaluate the relationship between hydrogen sulfide and hypoxia-inducible factor-1α (HIF-1α)during the osteogenesis of rat bone marrow mesenchymal cells under tensile stress.METHODS: 2000 μ strain was loaded on the in vitro cultured rat bone marrow mesenchymal cells by a four-point bending apparatus, and hydrogen sulfide donor or HIF-1α inhibitor was adopted in the tensile unit. Subsequently, the levels of osteogenic markers were detected.RESULTS AND CONCLUSION: Exogenous hydrogen sulfide signaling could promote the osteogenesis of rat bone marrow mesenchymal cells under tensile stress. However, this promotion was obviously eliminated when the endogenous HIF-1α expression was inhibited.These results show that hydrogen sulfide signaling system promotes the osteogenesis of rat bone marrow mesenchymal cells under tensile stress probably through HIF-1α.关键词
干细胞/骨髓干细胞/硫化氢/缺氧诱导因子/骨髓间充质细胞/张应力/大鼠/成骨/GYY4137/YC-1/牵张成骨/四点弯曲/国家自然科学基金分类
医药卫生引用本文复制引用
蒋校文,张翼,范晓升,陈彦泽..硫化氢通过缺氧诱导因子1α促进张应力下骨髓间充质细胞的成骨[J].中国组织工程研究,2017,21(21):3281-3286,6.基金项目
国家自然科学基金(81301651) （81301651）
郴州市第一人民医院重点项目(N2015-006)the National Natural Science Foundation of China, No. 81301651 （N2015-006）
the Key Project of the First People'sHospital of Chenzhou, No. N2015-006 （）
