中国组织工程研究2017，Vol.21Issue(21)：3293-3298,6.DOI:10.3969/j.issn.2095-4344.2017.21.003
当归多糖对小鼠骨髓造血干细胞及基质细胞表面细胞间黏附分子1水平的影响
Effect of angelica polysaccharide on bone marrow hematopoietic stem cells and intercellular adhesion molecule 1 on the surface of stromal cells in mice
摘要
Abstract
BACKGROUND:Angelica polysaccharide has the function of enriching blood and promoting blood circulation, but how to improve the hematopoietic microenvironment and promote hematopoiesis has become one of the hot topics. OBJECTIVE: To investigate the effect of angelica polysaccharide on Sca-1+, CD34+ hematopoietic stem cells and intercellular adhesion molecule 1 level in bone marrow stromal cells in mice. METHODS:Mouse models of hemorrhagic anemia were made and then randomly divided them into control group, low-concentration angelica polysaccharide group and high-concentration angelica polysaccharide group. Angelica polysaccharide groups were intraperitoneally injected with the corresponding concentration of angelica polysaccharide (4, 6 mg/kg) for continuous 6 days, while the control group was injected with equal amount of normal saline. General conditions of the model mice were observed. Peripheral blood red cells were counted by automatic blood cell analyzer. Bone marrow hematopoietic stem cels were counted by magnetic bead sorting technique and flow cytometry. Proliferation of bone marrow stromal cells was detected by MTT method. Expression of intercellular adhesion molecule-1 in bone marrow stromal cells was detected by flow cytometry. RESULTS AND CONCLUSION:Angelica polysaccharide had no obvious effect on the body mass of hemorrhagic anemia mice (P > 0.05). The number of peripheral blood cells, the percentage of bone marrow CD34+ and Sca-1+ cells, the number of bone marrow stromal cells and the level of intercellular adhesion molecule 1 were ranked as follows: high-concentration group > low-concentration group > control group (P < 0.05). To conclude, angelica polysaccharide could promote the proliferation of bone marrow stromal cells and increase the expression of intercellular adhesion molecule 1, thereby promoting the proliferation of hematopoietic stem cells and regulating their functional activities.关键词
干细胞/骨髓干细胞/当归多糖/造血干细胞/失血性贫血/骨髓/造血/微环境/基质细胞/细胞间黏附分子1/小鼠分类
医药卫生引用本文复制引用
王改琴,景鹏,贾书花..当归多糖对小鼠骨髓造血干细胞及基质细胞表面细胞间黏附分子1水平的影响[J].中国组织工程研究,2017,21(21):3293-3298,6.基金项目
长治医学院科技创新团队项目(CX201415) （CX201415）
山西省高校科技开发项目(20101101) the Innovation Team Project of Changzhi Medical College, No. CX201415 （20101101）
the Scientific and Technological Development Project of Shanxi Universities, No. 20101101 （）
