摘要
Abstract
BACKGROUND:With the improvement of cellular technology, stem cell transplantation has been involved in various diseases, such as transfusion-related acute lung injury (TRALI). OBJECTIVE:To investigate the protective effect of Tanreqing injection combined with human amniotic mesenchymal stem cells (hAMSCs) transplantation in a mouse model of TRALI. METHODS:A total of 80 BALB/C male mice were divided into control group, model group,Tanreqing injection group, hAMSCs transplantation group and combined group (Tanreqing injection+hAMSCs transplantation group). Animal models of TRALI were made in all groups except the control group. Mouse lung tissue pathology and wet/dry ratio were observed and calculated 24 hours after treatment. ELISA was used to measure interleukin-10, interleukin-1β, and tumor necrosis factor-α levels in bronchoalveolar lavage fluid. Expression of keratinocyte growth factor-1 mRNA and protein was detected by RT-PCR and western blot, respectively. Apoptosis in the lung tissue was detected by TUNEL method. RESULTS AND CONCLUSION:(1) Thickened alveolar septum, alveolar fibrin infiltration, alveolar hemorrhage, thickened bronchial wall, and increased lung wet/dry ratio were observed in the model group, and these pathological changes were slightly milder in theTanreqing injection group and hAMSCs transplantation group. Compared with the model group, the lung wet/dry ratio was significantly lower in theTanreqing injection group and hAMSCs transplantation group (P < 0.05), and very significantly lower in the combined group (P < 0.01). (2) Compared with the model group, the levels of interleukin-10, interleukin-1β, and tumor necrosis factor-α in the bronchoalveolar lavage fluid were significantly lower in theTanreqing injection group and hAMSCs transplantation group (P < 0.05), and very significantly lower in the combined group (P < 0.01), but these levels were still higher in the combined group than the control group (P < 0.05). (3) The expression levels of keratinocyte growth factor-1 mRNA and protein were ranked as follows: combined group >Tanreqing injection group and hAMSCs transplantation group > model group, and there were significant differences between them (P < 0.05). (4) The apoptotic index was ranked as folows: combined group <Tanreqing injection group and hAMSCs transplantation group < model group. To conclude,Tanreqing injection combined with hAMSCs transplantation can improve expression of keratinocyte growth factor-1 in the lung tissue of TRALI mice, reduce degree of lung inflammatory reaction and pathologic injury, and reduce the apoptosis in the lung tissue, which plays a protective role against lung injury in mice.关键词
干细胞/移植/人羊膜间充质干细胞/细胞移植/痰热清注射液/输血相关急性肺损伤/肺组织/角质细胞生长因子1/小鼠分类
医药卫生
