摘要
Abstract
[Objective] A high performance liquid chromatography (HPLC) method for the determination of contents of tartrazine , indigotin, sunset yellow, fancy red, brilliant blue, erythrosine, amaranth, new red,carmine is established in wine ; [Method] The HPLC separation was performed with the analysis column SB-Cl8 by using the mobile phase gradient elution of the 0.8mL/L methanol and 0.02mol/L ammonium acetate aqueous solution. Flow rate is 0.8mL/min, the wavelength of the detector - UV is 254nm. [Result] The method for the determination of 9 synthetic colorants exhibited excellent linearity over a range of 0.05 - 200mg/L (r > 0.9992), in addition 50mg/L level (n=3).The detection limit, The average recovery rate and relative standard deviation of this method was 0.0379 - 0.0756 mg/L, 82.4 ~ 94.6%, and 1.7 ~ 3.9%, respectively. 8 synthetic colorants were detected in Xinjiang real estate wine samples. the detection rate was 94.84%. The detection rate from high to low order sunset yellow> new red > carmine in wine > fancy red > amaranth >brilliant blue > tartrazine The detection range was 3.43 - 258.37. [Conclusion] this method is simple, rapid and accurate for the determination of synthetic colorants in wine ; There are synthetic colorants in the products of Xinjiang Wine.关键词
高效液相色谱法/葡萄酒/人工合成着色剂Key words
High performance liquid chromatographic method/wine/synthetic coloring agents分类
化学化工
