畜牧与兽医2017，Vol.49Issue(7)：46-49,4.
庆大霉素致原代大鼠肾小管上皮细胞凋亡的研究
Apoptosis of primary renal epithelial cells in rats induced by gentamicin
严磊 1张绘艳 1杨小康 1谢俊泽 1顾建红 1袁燕 1卞建春 1刘宗平 1刘学忠1
作者信息
- 1. 扬州大学兽医学院/江苏省动物重要疫病与人兽共患病防控协同创新中心,江苏扬州225009
- 折叠
摘要
Abstract
To investigate the apoptosis effects of gentamicin on primary culture of renal epithelial cells,primary culture of renal epithelial cells were treated with gentamicin (0～ 16 mmol/L) for 24 h.CCK-8 assay was used to determine the relative viability of cells.Flow cytometry (FCM) and hochest 33258 staining were used to detect apoptotic rate.The results showed that compared with the control group,the relative viability of cells was decreased and the apoptotic rate increased (P＜0.05 or P＜0.01),pyknosis or chromatin condensed after hochest 33258 staining as the (P＜ 0.01).The results suggested that gentamicin-induced cell apoptosis and maybe connected with gentamicininduced renal toxicity.关键词
庆大霉素/凋亡/肾小管上皮细胞Key words
gentamicin/apoptosis/renal epithelial cells分类
医药卫生引用本文复制引用
严磊,张绘艳,杨小康,谢俊泽,顾建红,袁燕,卞建春,刘宗平,刘学忠..庆大霉素致原代大鼠肾小管上皮细胞凋亡的研究[J].畜牧与兽医,2017,49(7):46-49,4.
