畜牧与兽医2017，Vol.49Issue(7)：69-73,5.
STING基因荧光定量检测方法的建立及其在鸭组织中的分布
Establishment of Real-time PCR for detection of STING gene and its tissue distribution in duck
摘要
Abstract
Two pairs of primers for duck STING gene were designed based on the mammal STING genes.The quantification of duck STING and its tissue distribution were investigated using Real-time quantitative reverse transcription PCR (qRT-PCR).This qRT-PCR was repeatable with the limit of detection as low as 4.8× 103 copies/μL,and absolute quantification linear range from 3.5× 10-5 to 0.27ng/μL.The duck STING mRNA was constitutively expressed in all tissues analyzed,with the highest levels in glandular stomach;higher levels in trachea,lung and small intestine;moderate levels in spleen,kidney,muscular stomach and liver;low levels in muscle and skin.The quantification method and tissue distribution determination of duck STING gene provide the foundation for further investigation of the role of duck STING in antiviral infection.关键词
鸭/STING基因/检测/荧光定量PCR/组织丰度Key words
duck/STING/detection/Real-time PCR/tissue distribution分类
农业科技引用本文复制引用
石树端,高全新,程玉强,刘云霞,孙建和,严亚贤..STING基因荧光定量检测方法的建立及其在鸭组织中的分布[J].畜牧与兽医,2017,49(7):69-73,5.基金项目
国家自然科学基金(31672524) （31672524）
