畜牧与兽医2017，Vol.49Issue(7)：74-78,5.
PRRSV上海浦东南汇分离株Nsp2与ORF5基因遗传演化分析
Geneticevolution analysis of NSP2 and ORF5 genes of porcine reproductive and respiratory syndrome virus from Nanhui in Pudong District of Shanghai
摘要
Abstract
Genetic evolution of porcine reproductive and respiratory syndrome virus (PRRSV) in Nanhui of Shanghai was investigated in order to provide the theoretical basis for prevention and control of PRRS.Clinical samples from some scale farms suspicious of PRRSV infection were collected.RNA was extracted and Nsp2 and ORF5 genes were amplified by PCR and sequenced.Sequence analysis revealed that all isolated strains of Nanhui had discontinuous 30 amino acid deletion in 481 aa and 533 to 561 aa in Nsp2 protein,and had the same multiple genomic variations in different regions with that of the highly pathogenicity PRRSV (HP-PRRSV) JXA1.Amino acid sequence comparison of Nsp2 and ORF5 demonstrated that the six strains shared 40.3％-55.3％,73.5％ ～ 89.5％;54.7％ ～ 75.1％,75.5％ ～ 92.5％;50.9％ ～ 67.3％,73.5％～90.5％;72.8％～91.5％,81.5％～98.5％ identities with VR-2332,CH-1a,HB-2 and JXA1,respectively.But there were 26.2％ ～ 35.9％ and 44.8％ ～ 56.4％ amino acid sequence identities in Nsp2 and ORFS,respectively,with European prototype Lelystad virus strain.Phylogenetic analysis showed that Nanhui strains located a relatively independent branch from domestic strains (CH-1 a,HB -2) and North American genotype VR-2332,but in the same evolutionary branch with JXA1 strains.The results indicated that the six PRRSV strains from Nanhui belong to North America HP-type and have certain geographical development feature.And these strains have been independent from the tree of PRRSV genetic evolution and form a new genetic group.关键词
猪繁殖与呼吸综合征病毒/鉴定/Nsp2/ORF5/序列分析Key words
porcine reproductive and respiratory syndrome virus/identification/Nsp2/ORFS/sequence analysis分类
农业科技引用本文复制引用
郁星星,朱爱军,邢涛,王海根,张红飞,周光胜,李金贵..PRRSV上海浦东南汇分离株Nsp2与ORF5基因遗传演化分析[J].畜牧与兽医,2017,49(7):74-78,5.基金项目
上海市科技兴农推广项目[沪农科推字(2015)第1-5号] （2015）
